Facile, rapid and efficient biofabrication of gold nanoparticles decorated with functional proteinsElectronic supplementary information (ESI) available. See DOI: 10.1039/c2an35172b

We report a one-pot biological approach to fabricate gold nanoparticle (AuNP)-ZZ domain conjugates using peptide-functionalized proteins that can simultaneously direct both biomineralization and surface modification of AuNPs. In addition, immuno-AuNPs are readily prepared through the specific binding of antibodies to the ZZ domain on the AuNPs. We have developed a simple one-pot strategy to fabricate highly stabilized, monodisperse gold nanoparticle-protein conjugates that can be readily derivatized with antibodies.