Coherent two-dimensional infrared spectroscopy: Quantitative analysis of protein secondary structure in solution

We present a method to quantitatively determine the secondary structure composition of globular proteins using coherent two-dimensional infrared (2DIR) spectroscopy of backbone amide I vibrations (15501720 cm 1 ). Sixteen proteins with known crystal structures were used to construct a library of 2DIR spectra, and the fraction of residues in -helix, -sheet, and unassigned conformations was determined by singular value decomposition (SVD) of the measured two-dimensional spectra. The method was benchmarked by removing each individual protein from the set and comparing the composition extracted from 2DIR against the composition determined from the crystal structures. To highlight the increased structural content extracted from 2DIR spectra a similar analysis was also carried out using conventional infrared absorption of the proteins in the library. We present a new spectroscopic technique to measure the fraction of protein residues in alpha-helix, beta-sheet, and unstructured conformations.