A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole bloodElectronic supplementary information (ESI) available: Figures S1-S7, Tables S1 and S2, Videos S1-S6, and Methods. See DOI: 10.1039/c0lc00132e

We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay sur...

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Hauptverfasser: Wang, Jun, Ahmad, Habib, Ma, Chao, Shi, Qihui, Vermesh, Ophir, Vermesh, Udi, Heath, James
Format: Artikel
Sprache:eng
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Zusammenfassung:We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody "barcode" arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings. A filter paper powered chip, designed for point-of-care diagnostics, is demonstrated for sequentially and automatically executing all steps required for a quantitative, multiplex protein assay from a pin prick of whole blood
ISSN:1473-0197
1473-0189
DOI:10.1039/c0lc00132e