Inverting the enantioselectivity of P450pyr monooxygenase by directed evolutionThis article is part of the 'Enzymes and Proteins' web-theme issue for ChemComm.Electronic supplementary information (ESI) available: Materials and experimental procedures. See DOI: 10.1039/c0cc00735h

We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% ( R ) compared to the wild type's ee of 43% ( S ). The first example of directed evolution of a novel P450pyr monooxygenase with inverted enantioselectivity has been demonstrated.