Eukaryotic DNA Methylases and Their Use for in vitro Methylation

DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, w...

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Veröffentlicht in:	Philosophical transactions of the Royal Society of London. Series B, Biological sciences Biological sciences, 1990-01, Vol.326 (1235), p.189-198
Hauptverfasser:	Adams, R. L. P., Bryans, M., Rinaldi, A., Smart, A., Yesufu, H. M. I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Ascites Base Sequence Biological and medical sciences Chromatin Chromatography, Affinity Chromatography, Gel DNA DNA Modification Methylases - isolation & purification DNA Modification Methylases - metabolism Enzymes Enzymes and enzyme inhibitors Fabaceae - enzymology Fundamental and applied biological sciences. Psychology HeLa cells Kinetics Liver Liver - enzymology Methylation Mice Molecular Sequence Data Molecular Weight Molecules Peas Plants, Medicinal Plasmids Substrate Specificity Transferases
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Beschreibung
Zusammenfassung:	DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, which makes it difficult to saturate all possible sites on the DNA, but procedures are described that circumvent this problem.
ISSN:	0962-8436 0080-4622 1471-2970 2054-0280
DOI:	10.1098/rstb.1990.0003