Intact endothelial cell sheet harvesting from thermoresponsive surfaces coated with cell adhesion promoters

-isopropylacrylamide) (PNIPAAm). However, this requires expensive techniques to generate a sufficiently thin film that allows cell adhesion. In this study, we looked at simple solvent cast films which normally show poor cell adhesion, but here the films are coated with cell adhesion promoters (CAPs)...

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Veröffentlicht in:	Journal of the Royal Society interface 2007-12, Vol.4 (17), p.1151-1157
Hauptverfasser:	Moran, Martin T, Carroll, William M, Gorelov, Alexander, Rochev, Yuri
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamides - chemistry Atomic Force Microscopy Biocompatible Materials - chemistry Cell Adhesion - drug effects Cell Adhesion Promoters Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Detachment Cell Proliferation Cells, Cultured Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - physiology Hot Temperature Humans Isopropylacrylamide Poly poly(N-isopropylacrylamide) Polymers - chemistry Surface Properties Temperature Time Factors
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Beschreibung
Zusammenfassung:	-isopropylacrylamide) (PNIPAAm). However, this requires expensive techniques to generate a sufficiently thin film that allows cell adhesion. In this study, we looked at simple solvent cast films which normally show poor cell adhesion, but here the films are coated with cell adhesion promoters (CAPs) to improve cell growth without altering the copolymer thermoresponsive behaviour.
ISSN:	1742-5689 1742-5662
DOI:	10.1098/rsif.2007.1023