A new allele PEL9 GG identified by genome-wide association study increases panicle elongation length in rice ( Oryza sativa L.)

Considering the male sterile line has the phenomenon of panicle enclosure, panicle elongation length (PEL) plays an important role in hybrid rice seed production. However, the molecular mechanism underlying this process is poorly understood. In this study, we investigated the PEL phenotypic values of 353 rice accessions across six environments, which shows abundant phenotypic variation. Combining the 1.3 million single-nucleotide polymorphisms, we performed a genome-wide association study on PEL. Three quantitative trait loci (QTL) , , and were identified as significantly associated with PEL, of which and were previously reported QTLs and was novel. One causal gene locus, , was identified and validated. The PEL of accessions carrying allele was significantly longer than that of those carrying allele . We also demonstrated that the outcrossing rate of female parents carrying allele increased by 14.81% compared with that of the isogenic line carrying allele in an F hybrid seed production field. The allele frequency of increased gradually with an increase in latitude in the Northern Hemisphere. Our results should facilitate the improvement of the PEL of the female parent of hybrid rice.