Characterization, Physical Properties, and Biocompatibility of Novel Tricalcium Silicate–Chitosan Endodontic Sealer

Abstract Objective The purpose of this study was to compare the characteristics, physical properties, and biocompatibility of the novel tricalcium silicate–chitosan (TCS-C) sealer with AH Plus and Sure-Seal Root. Materials and Methods The TCS-C powder was prepared by mixing tricalcium silicate with 2% water-soluble chitosan at a 5:1 ratio, followed by sufficient addition of 10 g/mL ratio of double-distilled water to form a homogeneous cement. Material characterizations (the Fourier Transform InfraRed [FTIR] and X-ray diffraction [XRD]), physical property investigations (flow and film thickness), and cytotoxicity tests in 3T3 mouse embryo fibroblast cell (MTT assay method) were performed on sealers, and the results were compared with those of the commercial products. Statistical Analysis Statistical analysis was performed on flow and film thickness. The normality of the data was tested using the Shapiro–Wilk test. Statistical analysis was performed with one-way analysis of variance (ANOVA). The level of significance was set at p