Comprehensive Analysis and Functional Verification of the Pinus massoniana NBS-LRR Gene Family Involved in the Resistance to Bursaphelenchus xylophilus

Lamb. is a crucial timber and resin conifer in China, but its plantation industry is threatened by outbreaks of pine wilt disease (PWD) caused by (pinewood nematode; PWN). However, as of yet, there is no comprehensive analysis of NBS-LRR genes in involved in its defense against PWN. In this study, 507 NBS genes were identified in the transcriptome of resistant and susceptible inoculated with the PWN. The phylogenetic analysis and expression profiles of resistant and susceptible revealed that the up-regulated gene was involved in conferring resistance to PWN. The results of real-time quantitative PCR (qRT-PCR) showed that was significantly up-regulated after PWN infection, especially in the stems. Subcellular localization indicated that PmNBS-LRR97 located to the cell membrane. significantly activated the expression of reactive oxygen species (ROS)-related genes in . In addition, the overexpression of was capable of promoting the production of ROS, aiding in plant growth and development. In summary, participates in the defense response to PWN and plays an active role in conferring resistance in . This finding provides new insight into the regulatory mechanism of the R gene in .