MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2
Lung adenocarcinoma (LUAD) is the most common type of lung cancer, and its pathogenesis is still unclear. The present study aimed to investigate the role of and its downstream target gene, ribonucleotide reductase regulatory subunit M2 ( ), in the occurrence and development of LUAD and elucidate the...
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| Veröffentlicht in: | Annals of translational medicine 2022-12, Vol.10 (24), p.1374-1374 |
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| creator | Cao, Xiaowen Xue, Fangsu Chen, Haoyu Shen, Lu Yuan, Xiaosa Yu, Yunchi Zong, Ying Zhong, Lou Huang, Fang |
| description | Lung adenocarcinoma (LUAD) is the most common type of lung cancer, and its pathogenesis is still unclear. The present study aimed to investigate the role of
and its downstream target gene, ribonucleotide reductase regulatory subunit M2 (
), in the occurrence and development of LUAD and elucidate the correlation between
and the clinicopathological stage and prognosis of LUAD.
The expression of
was analyzed using the CancerMIRNome database and quantitative polymerase chain reaction (qPCR). The effects of
and
on the proliferation, migration, and invasion of A549 cells were analyzed. A dual luciferase reporter assay was used to verify the targeting of
and
. Additionally, the correlation between
expression and clinicopathology was analyzed.
(I)
was lowly expressed in LUAD and the LUAD cell lines. qPCR confirmed that microRNA (miRNA) transfection was effective and sufficient for subsequent experiments. (II)
inhibited the proliferation, invasion, and migration of LUAD cells. (III) There was a targeting relationship between
and
, and
affected the expression of the
protein.
was highly expressed in lung cancer tissue. (IV)
was associated with the clinicopathological staging of lung cancer. The prognosis of patients with low
expression was better, and the prognostic sensitivity of
to lung cancer was high.
may exert its effects via the Notch pathway. (V) Si-
inhibited the expression of the
protein.
promoted the proliferation, migration, and invasion of LUAD cells. A
inhibitor restored the inhibitory effect of si-
on LUAD cells.
was lowly expressed in lung cancer tissue.
overexpression inhibited the proliferation and metastasis of lung cancer cells.
was highly expressed in lung cancer tissue and promoted the proliferation and metastasis of lung cancer cells.
targeted and inhibited
, thereby reducing the proliferation and metastasis of LUAD cells. LUAD patients with low
expression had a better prognosis, and the expression level of
was correlated with the clinical characteristics of lung cancer patients. |
| doi_str_mv | 10.21037/atm-22-6089 |
| format | Article |
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and its downstream target gene, ribonucleotide reductase regulatory subunit M2 (
), in the occurrence and development of LUAD and elucidate the correlation between
and the clinicopathological stage and prognosis of LUAD.
The expression of
was analyzed using the CancerMIRNome database and quantitative polymerase chain reaction (qPCR). The effects of
and
on the proliferation, migration, and invasion of A549 cells were analyzed. A dual luciferase reporter assay was used to verify the targeting of
and
. Additionally, the correlation between
expression and clinicopathology was analyzed.
(I)
was lowly expressed in LUAD and the LUAD cell lines. qPCR confirmed that microRNA (miRNA) transfection was effective and sufficient for subsequent experiments. (II)
inhibited the proliferation, invasion, and migration of LUAD cells. (III) There was a targeting relationship between
and
, and
affected the expression of the
protein.
was highly expressed in lung cancer tissue. (IV)
was associated with the clinicopathological staging of lung cancer. The prognosis of patients with low
expression was better, and the prognostic sensitivity of
to lung cancer was high.
may exert its effects via the Notch pathway. (V) Si-
inhibited the expression of the
protein.
promoted the proliferation, migration, and invasion of LUAD cells. A
inhibitor restored the inhibitory effect of si-
on LUAD cells.
was lowly expressed in lung cancer tissue.
overexpression inhibited the proliferation and metastasis of lung cancer cells.
was highly expressed in lung cancer tissue and promoted the proliferation and metastasis of lung cancer cells.
targeted and inhibited
, thereby reducing the proliferation and metastasis of LUAD cells. LUAD patients with low
expression had a better prognosis, and the expression level of
was correlated with the clinical characteristics of lung cancer patients.</description><identifier>ISSN: 2305-5839</identifier><identifier>EISSN: 2305-5839</identifier><identifier>DOI: 10.21037/atm-22-6089</identifier><identifier>PMID: 36660663</identifier><language>eng</language><publisher>China: AME Publishing Company</publisher><subject>Original</subject><ispartof>Annals of translational medicine, 2022-12, Vol.10 (24), p.1374-1374</ispartof><rights>2022 Annals of Translational Medicine. All rights reserved.</rights><rights>2022 Annals of Translational Medicine. All rights reserved. 2022 Annals of Translational Medicine.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-e7e89a7860cbb63b2c8564ab0893ce82924a423df3af34c2fad1d80f70fce3e73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843311/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843311/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36660663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Xiaowen</creatorcontrib><creatorcontrib>Xue, Fangsu</creatorcontrib><creatorcontrib>Chen, Haoyu</creatorcontrib><creatorcontrib>Shen, Lu</creatorcontrib><creatorcontrib>Yuan, Xiaosa</creatorcontrib><creatorcontrib>Yu, Yunchi</creatorcontrib><creatorcontrib>Zong, Ying</creatorcontrib><creatorcontrib>Zhong, Lou</creatorcontrib><creatorcontrib>Huang, Fang</creatorcontrib><title>MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2</title><title>Annals of translational medicine</title><addtitle>Ann Transl Med</addtitle><description>Lung adenocarcinoma (LUAD) is the most common type of lung cancer, and its pathogenesis is still unclear. The present study aimed to investigate the role of
and its downstream target gene, ribonucleotide reductase regulatory subunit M2 (
), in the occurrence and development of LUAD and elucidate the correlation between
and the clinicopathological stage and prognosis of LUAD.
The expression of
was analyzed using the CancerMIRNome database and quantitative polymerase chain reaction (qPCR). The effects of
and
on the proliferation, migration, and invasion of A549 cells were analyzed. A dual luciferase reporter assay was used to verify the targeting of
and
. Additionally, the correlation between
expression and clinicopathology was analyzed.
(I)
was lowly expressed in LUAD and the LUAD cell lines. qPCR confirmed that microRNA (miRNA) transfection was effective and sufficient for subsequent experiments. (II)
inhibited the proliferation, invasion, and migration of LUAD cells. (III) There was a targeting relationship between
and
, and
affected the expression of the
protein.
was highly expressed in lung cancer tissue. (IV)
was associated with the clinicopathological staging of lung cancer. The prognosis of patients with low
expression was better, and the prognostic sensitivity of
to lung cancer was high.
may exert its effects via the Notch pathway. (V) Si-
inhibited the expression of the
protein.
promoted the proliferation, migration, and invasion of LUAD cells. A
inhibitor restored the inhibitory effect of si-
on LUAD cells.
was lowly expressed in lung cancer tissue.
overexpression inhibited the proliferation and metastasis of lung cancer cells.
was highly expressed in lung cancer tissue and promoted the proliferation and metastasis of lung cancer cells.
targeted and inhibited
, thereby reducing the proliferation and metastasis of LUAD cells. LUAD patients with low
expression had a better prognosis, and the expression level of
was correlated with the clinical characteristics of lung cancer patients.</description><subject>Original</subject><issn>2305-5839</issn><issn>2305-5839</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpVkcFLHTEQxkNpqWK99Vxy9NDVZGZfNnspiFgVlMKjPYfZbPJeym7ymuQJ_vddqxWFgRmYH9_Mx8fYZylOQQrszqjODUCjhO7fsUNAsWpWGvv3r-YDdlzKbyGEBNmjEB_ZASqlhFJ4yOxdWDcgoMEdD3EbhlALr1vHdzlNwbtMNaTIKY58dpXKUqHw5Pm0jxtOo4vJUrYhppm4ddNU-PDAK-WNq2Eh1us7-MQ-eJqKO37uR-zX98ufF9fN7Y-rm4vz28aibGvjOqd76rQSdhgUDmD1SrU0LNbQOg09tNQCjh7JY2vB0yhHLXwnvHXoOjxi3550d_thdqN1sWaazC6HmfKDSRTM200MW7NJ96bXLaKUi8DJs0BOf_auVDOH8miKokv7YqBTGhBA4IJ-fUJtTqVk51_OSGH-RWOWaAyAeYxmwb-8fu0F_h8E_gXELIsm</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Cao, Xiaowen</creator><creator>Xue, Fangsu</creator><creator>Chen, Haoyu</creator><creator>Shen, Lu</creator><creator>Yuan, Xiaosa</creator><creator>Yu, Yunchi</creator><creator>Zong, Ying</creator><creator>Zhong, Lou</creator><creator>Huang, Fang</creator><general>AME Publishing Company</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20221201</creationdate><title>MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2</title><author>Cao, Xiaowen ; Xue, Fangsu ; Chen, Haoyu ; Shen, Lu ; Yuan, Xiaosa ; Yu, Yunchi ; Zong, Ying ; Zhong, Lou ; Huang, Fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-e7e89a7860cbb63b2c8564ab0893ce82924a423df3af34c2fad1d80f70fce3e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Original</topic><toplevel>online_resources</toplevel><creatorcontrib>Cao, Xiaowen</creatorcontrib><creatorcontrib>Xue, Fangsu</creatorcontrib><creatorcontrib>Chen, Haoyu</creatorcontrib><creatorcontrib>Shen, Lu</creatorcontrib><creatorcontrib>Yuan, Xiaosa</creatorcontrib><creatorcontrib>Yu, Yunchi</creatorcontrib><creatorcontrib>Zong, Ying</creatorcontrib><creatorcontrib>Zhong, Lou</creatorcontrib><creatorcontrib>Huang, Fang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Xiaowen</au><au>Xue, Fangsu</au><au>Chen, Haoyu</au><au>Shen, Lu</au><au>Yuan, Xiaosa</au><au>Yu, Yunchi</au><au>Zong, Ying</au><au>Zhong, Lou</au><au>Huang, Fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2</atitle><jtitle>Annals of translational medicine</jtitle><addtitle>Ann Transl Med</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>10</volume><issue>24</issue><spage>1374</spage><epage>1374</epage><pages>1374-1374</pages><issn>2305-5839</issn><eissn>2305-5839</eissn><abstract>Lung adenocarcinoma (LUAD) is the most common type of lung cancer, and its pathogenesis is still unclear. The present study aimed to investigate the role of
and its downstream target gene, ribonucleotide reductase regulatory subunit M2 (
), in the occurrence and development of LUAD and elucidate the correlation between
and the clinicopathological stage and prognosis of LUAD.
The expression of
was analyzed using the CancerMIRNome database and quantitative polymerase chain reaction (qPCR). The effects of
and
on the proliferation, migration, and invasion of A549 cells were analyzed. A dual luciferase reporter assay was used to verify the targeting of
and
. Additionally, the correlation between
expression and clinicopathology was analyzed.
(I)
was lowly expressed in LUAD and the LUAD cell lines. qPCR confirmed that microRNA (miRNA) transfection was effective and sufficient for subsequent experiments. (II)
inhibited the proliferation, invasion, and migration of LUAD cells. (III) There was a targeting relationship between
and
, and
affected the expression of the
protein.
was highly expressed in lung cancer tissue. (IV)
was associated with the clinicopathological staging of lung cancer. The prognosis of patients with low
expression was better, and the prognostic sensitivity of
to lung cancer was high.
may exert its effects via the Notch pathway. (V) Si-
inhibited the expression of the
protein.
promoted the proliferation, migration, and invasion of LUAD cells. A
inhibitor restored the inhibitory effect of si-
on LUAD cells.
was lowly expressed in lung cancer tissue.
overexpression inhibited the proliferation and metastasis of lung cancer cells.
was highly expressed in lung cancer tissue and promoted the proliferation and metastasis of lung cancer cells.
targeted and inhibited
, thereby reducing the proliferation and metastasis of LUAD cells. LUAD patients with low
expression had a better prognosis, and the expression level of
was correlated with the clinical characteristics of lung cancer patients.</abstract><cop>China</cop><pub>AME Publishing Company</pub><pmid>36660663</pmid><doi>10.21037/atm-22-6089</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| title | MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2 |
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