Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes

Background CRISPR/Cas9 is the most powerful and versatile genome-editing tool that permits multiplexed-targeted gene modifications for the genetic enhancement of oil palm. Multiplex genome-editing has recently been developed for modifying multiple loci in a gene or multiple genes in a genome with hi...

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Veröffentlicht in:	Journal of Genetic Engineering and Biotechnology 2023-12, Vol.21 (1), p.3-18, Article 3
Hauptverfasser:	Bahariah, Bohari, Masani, Mat Yunus Abdul, Fizree, Md Piji Mohd Al Akmarul, Rasid, Omar Abd, Parveez, Ghulam Kadir Ahmad
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyl carrier protein biolistics Biomedical Engineering and Bioengineering Biosynthesis biotechnology callus computer simulation CRISPR CRISPR-Cas systems CRISPR/Cas9 Desaturase Editing EgFAD2 EgPAT Elaeis guineensis Engineering Enzymes Expression vectors fatty acid desaturase fatty acid metabolism Fatty acids gene editing Genes Genetic aspects Genetic engineering genetic improvement Genetic modification Genetic research Genetic transformation Genetically modified organisms Genome editing Genomes Genomics Monounsaturated fatty acids Multiplexing Mutation Oil palm oils Oils & fats Oleic acid protoplasts RNA polymerase Sequence analysis Vegetable oils
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