Genomic features of Helicobacter pylori‐naïve diffuse‐type gastric cancer

Helicobacter pylori (HP) is a major etiologic driver of diffuse‐type gastric cancer (DGC). However, improvements in hygiene have led to an increase in the prevalence of HP‐naïve DGC; that is, DGC that occurs independent of HP. Although multiple genomic cohort studies for gastric cancer have been conducted, including studies for DGC, distinctive genomic differences between HP‐exposed and HP‐naïve DGC remain largely unknown. Here, we employed exome and RNA sequencing with immunohistochemical analyses to perform binary comparisons between 36 HP‐exposed and 27 HP‐naïve DGCs from sporadic, early‐stage, and intramucosal or submucosal tumor samples. Among the samples, 33 HP‐exposed and 17 HP‐naïve samples had been preserved as fresh‐frozen samples. HP infection status was determined using stringent criteria. HP‐exposed DGCs exhibited an increased single nucleotide variant burden (HP‐exposed DGCs; 1.97 [0.48–7.19] and HP‐naïve DGCs; 1.09 [0.38–3.68] per megabase; p = 0.0003) and a higher prevalence of chromosome arm‐level aneuploidies (p