Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice
This study aimed to examine the effect of Debaryomyces hansenii CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two D. hansenii (Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. S...
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| creator | Angulo, Miriam Ramos, Abel Reyes-Becerril, Martha Guerra, Kevyn Monreal-Escalante, Elizabeth Angulo, Carlos |
| description | This study aimed to examine the effect of
Debaryomyces hansenii
CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two
D. hansenii
(Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7 and 15. Mouse splenocytes were isolated and challenged with
Escherichia coli
. Immunological assays and immune-related gene expressions were performed. Serum was obtained from blood for total IgA and IgG antibody titer determination. Gut samples were taken for yeast colonization assessment. Phagocytosis, respiratory burst activity, and nitric oxide production in mice were mainly enhanced (
p
|
| doi_str_mv | 10.1007/s13205-022-03442-6 |
| format | Article |
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Debaryomyces hansenii
CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two
D. hansenii
(Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7 and 15. Mouse splenocytes were isolated and challenged with
Escherichia coli
. Immunological assays and immune-related gene expressions were performed. Serum was obtained from blood for total IgA and IgG antibody titer determination. Gut samples were taken for yeast colonization assessment. Phagocytosis, respiratory burst activity, and nitric oxide production in mice were mainly enhanced (
p
< 0.05) upon 7 days of
D. hansenii
intake at a concentration of 10ˆ8 CFU before and after bacterial challenge. Moreover, oral
D. hansenii
in mice upregulated (
p
< 0.05) gene expression of pro-inflammatory cytokines (INF-γ, IL-6 and IL-1β) before or after
E. coli
challenge on day 7 but downregulated (
p
< 0.05) on day 15. Furthermore, total serum IgG and IgA titers were higher (
p
< 0.05) in Dh 10ˆ8 CFU at days 7 and 15, and only at day 7, respectively, than that in the other dose and control groups. Finally,
D. hansenii
was detected in the gut of mice that received the treatments, suggesting that yeast survived gastrointestinal transit. Altogether, a short period (7 days) of
D. hansenii
CBS 8339 oral delivery improved immune innate response on mice.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-022-03442-6</identifier><identifier>PMID: 36590244</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Bioinformatics ; Biomaterials ; Biotechnology ; Blood ; Cancer Research ; Chemistry ; Chemistry and Materials Science ; Debaryomyces hansenii ; Digestive system ; E coli ; Gastrointestinal tract ; Gene expression ; IgG antibody ; IL-1β ; Immune response ; Immunoglobulin A ; Immunoglobulin G ; Immunology ; Inflammation ; Innate immunity ; Interleukin 6 ; Nitric oxide ; Original ; Original Article ; Phagocytosis ; Probiotics ; Respiratory burst ; Splenocytes ; Stem Cells ; Yeast</subject><ispartof>3 Biotech, 2023-01, Vol.13 (1), p.28-28, Article 28</ispartof><rights>King Abdulaziz City for Science and Technology 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>King Abdulaziz City for Science and Technology 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-d6bc2a801689d8b6a580a5477d1dd8d7a1a72e97f202af144c102df9aa080ee83</citedby><cites>FETCH-LOGICAL-c430t-d6bc2a801689d8b6a580a5477d1dd8d7a1a72e97f202af144c102df9aa080ee83</cites><orcidid>0000-0002-7965-1679</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797638/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797638/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,41486,42555,51317,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36590244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angulo, Miriam</creatorcontrib><creatorcontrib>Ramos, Abel</creatorcontrib><creatorcontrib>Reyes-Becerril, Martha</creatorcontrib><creatorcontrib>Guerra, Kevyn</creatorcontrib><creatorcontrib>Monreal-Escalante, Elizabeth</creatorcontrib><creatorcontrib>Angulo, Carlos</creatorcontrib><title>Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>This study aimed to examine the effect of
Debaryomyces hansenii
CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two
D. hansenii
(Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7 and 15. Mouse splenocytes were isolated and challenged with
Escherichia coli
. Immunological assays and immune-related gene expressions were performed. Serum was obtained from blood for total IgA and IgG antibody titer determination. Gut samples were taken for yeast colonization assessment. Phagocytosis, respiratory burst activity, and nitric oxide production in mice were mainly enhanced (
p
< 0.05) upon 7 days of
D. hansenii
intake at a concentration of 10ˆ8 CFU before and after bacterial challenge. Moreover, oral
D. hansenii
in mice upregulated (
p
< 0.05) gene expression of pro-inflammatory cytokines (INF-γ, IL-6 and IL-1β) before or after
E. coli
challenge on day 7 but downregulated (
p
< 0.05) on day 15. Furthermore, total serum IgG and IgA titers were higher (
p
< 0.05) in Dh 10ˆ8 CFU at days 7 and 15, and only at day 7, respectively, than that in the other dose and control groups. Finally,
D. hansenii
was detected in the gut of mice that received the treatments, suggesting that yeast survived gastrointestinal transit. Altogether, a short period (7 days) of
D. hansenii
CBS 8339 oral delivery improved immune innate response on mice.</description><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Blood</subject><subject>Cancer Research</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Debaryomyces hansenii</subject><subject>Digestive system</subject><subject>E coli</subject><subject>Gastrointestinal tract</subject><subject>Gene expression</subject><subject>IgG antibody</subject><subject>IL-1β</subject><subject>Immune response</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Innate immunity</subject><subject>Interleukin 6</subject><subject>Nitric oxide</subject><subject>Original</subject><subject>Original Article</subject><subject>Phagocytosis</subject><subject>Probiotics</subject><subject>Respiratory burst</subject><subject>Splenocytes</subject><subject>Stem Cells</subject><subject>Yeast</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kUuLFDEUhYMoztDOH3AhATduSm8eValsBG11RhhwQAV3IZXcmsnQlbRJ1UD_e9P22D4WZpPA-e5JTg4hTxm8ZADqVWGCQ9sA5w0IKXnTPSCnnGloWiX6h8cz_3ZCzkq5hbpa1moGj8mJ6FoNXMpTcnWV0xDSHBx9h4PNuzTtHBZ6Y2PBGAJdv_1MeyE03aEtM8VYFYeehmlaItKMZZsqWmiIdAoOn5BHo90UPLvfV-Trh_df1hfN5afzj-s3l42TAubGd4PjtgfW9dr3Q2fbHmwrlfLM-94ry6ziqNXIgduRSekYcD9qa6EHxF6syOuD73YZJvQO45ztxmxzmGoKk2wwfysx3JjrdGe00qoTe4MX9wY5fV-wzGYKxeFmYyOmpRiuOmAKZA2_Is__QW_TkmONV6n9TzLQe0N-oFxOpWQcj49hYPadmUNnpnZmfnZmujr07M8Yx5FfDVVAHIBSpXiN-ffd_7H9AWKtoVY</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Angulo, Miriam</creator><creator>Ramos, Abel</creator><creator>Reyes-Becerril, Martha</creator><creator>Guerra, Kevyn</creator><creator>Monreal-Escalante, Elizabeth</creator><creator>Angulo, Carlos</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7965-1679</orcidid></search><sort><creationdate>20230101</creationdate><title>Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice</title><author>Angulo, Miriam ; Ramos, Abel ; Reyes-Becerril, Martha ; Guerra, Kevyn ; Monreal-Escalante, Elizabeth ; Angulo, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-d6bc2a801689d8b6a580a5477d1dd8d7a1a72e97f202af144c102df9aa080ee83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agriculture</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Blood</topic><topic>Cancer Research</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Debaryomyces hansenii</topic><topic>Digestive system</topic><topic>E coli</topic><topic>Gastrointestinal tract</topic><topic>Gene expression</topic><topic>IgG antibody</topic><topic>IL-1β</topic><topic>Immune response</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin G</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Innate immunity</topic><topic>Interleukin 6</topic><topic>Nitric oxide</topic><topic>Original</topic><topic>Original Article</topic><topic>Phagocytosis</topic><topic>Probiotics</topic><topic>Respiratory burst</topic><topic>Splenocytes</topic><topic>Stem Cells</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angulo, Miriam</creatorcontrib><creatorcontrib>Ramos, Abel</creatorcontrib><creatorcontrib>Reyes-Becerril, Martha</creatorcontrib><creatorcontrib>Guerra, Kevyn</creatorcontrib><creatorcontrib>Monreal-Escalante, Elizabeth</creatorcontrib><creatorcontrib>Angulo, Carlos</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angulo, Miriam</au><au>Ramos, Abel</au><au>Reyes-Becerril, Martha</au><au>Guerra, Kevyn</au><au>Monreal-Escalante, Elizabeth</au><au>Angulo, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>13</volume><issue>1</issue><spage>28</spage><epage>28</epage><pages>28-28</pages><artnum>28</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>This study aimed to examine the effect of
Debaryomyces hansenii
CBS 8339 on innate immune responses in mice. Thirty BALB/c mice were randomly treated with phosphate buffered saline (PBS) (control) and two
D. hansenii
(Dh) doses: Dh 10ˆ6 CFU (colony forming units) and Dh 10ˆ8 CFU daily for 15 days. Spleen, blood, and gut samples were taken on days 7 and 15. Mouse splenocytes were isolated and challenged with
Escherichia coli
. Immunological assays and immune-related gene expressions were performed. Serum was obtained from blood for total IgA and IgG antibody titer determination. Gut samples were taken for yeast colonization assessment. Phagocytosis, respiratory burst activity, and nitric oxide production in mice were mainly enhanced (
p
< 0.05) upon 7 days of
D. hansenii
intake at a concentration of 10ˆ8 CFU before and after bacterial challenge. Moreover, oral
D. hansenii
in mice upregulated (
p
< 0.05) gene expression of pro-inflammatory cytokines (INF-γ, IL-6 and IL-1β) before or after
E. coli
challenge on day 7 but downregulated (
p
< 0.05) on day 15. Furthermore, total serum IgG and IgA titers were higher (
p
< 0.05) in Dh 10ˆ8 CFU at days 7 and 15, and only at day 7, respectively, than that in the other dose and control groups. Finally,
D. hansenii
was detected in the gut of mice that received the treatments, suggesting that yeast survived gastrointestinal transit. Altogether, a short period (7 days) of
D. hansenii
CBS 8339 oral delivery improved immune innate response on mice.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>36590244</pmid><doi>10.1007/s13205-022-03442-6</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7965-1679</orcidid><oa>free_for_read</oa></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Bioinformatics Biomaterials Biotechnology Blood Cancer Research Chemistry Chemistry and Materials Science Debaryomyces hansenii Digestive system E coli Gastrointestinal tract Gene expression IgG antibody IL-1β Immune response Immunoglobulin A Immunoglobulin G Immunology Inflammation Innate immunity Interleukin 6 Nitric oxide Original Original Article Phagocytosis Probiotics Respiratory burst Splenocytes Stem Cells Yeast |
| title | Probiotic Debaryomyces hansenii CBS 8339 yeast enhanced immune responses in mice |
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