GlPS1 overexpression accumulates coumarin secondary metabolites in transgenic Arabidopsis
The dried root of Glehnia littoralis is a traditional Chinese herbal medicine mainly used to treat lung diseases and plays an important role in fighting coronavirus disease 2019 pneumonia in China. This study focused on the key enzyme gene GlPS1 for furanocoumarin synthesis in G. littoralis . In the...
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Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2023-03, Vol.152 (3), p.539-553 |
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Sprache: | eng |
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Zusammenfassung: | The dried root of
Glehnia littoralis
is a traditional Chinese herbal medicine mainly used to treat lung diseases and plays an important role in fighting coronavirus disease 2019 pneumonia in China. This study focused on the key enzyme gene
GlPS1
for furanocoumarin synthesis in
G. littoralis
. In the 35S:
GlPS1
transgenic
Arabidopsis
study, the
Arabidopsis thaliana
-overexpressing
GlPS1
gene was more salt-tolerant than
Arabidopsis
in the blank group. Metabolomics analysis showed 30 differential metabolites in
Arabidopsis
, which overexpressed the
GlPS1
gene. Twelve coumarin compounds were significantly upregulated, and six of these coumarin compounds were not detected in the blank group. Among these differential coumarin metabolites, isopimpinellin and aesculetin have been annotated by the Kyoto Encyclopedia of Genes and Genomes and isopimpinellin was not detected in the blank group. Through structural comparison, imperatorin was formed by dehydration and condensation of zanthotoxol and a molecule of isoprenol, and the difference between them was only one isoprene. Results showed that the
GlPS1
gene positively regulated the synthesis of coumarin metabolites in
A. thaliana
and at the same time improved the salt tolerance of
A. thaliana
.
Key message
The results demonstrated that
GlPS1
positively regulates the biosynthetic pathway of furacoumarin, resulting in the accumulation of coumarin metabolites in transgenic
Arabidopsis thaliana
and the enhanced tolerance to salt stress. |
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ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-022-02427-w |