Protocol for investigating the biogenesis of SARS-CoV2-S pseudoviruses in HEK293T cells transduced to express the virus-specific intrabodies

The protocol investigates the influence of anti-cleavage site intrabodies in modulating the output of LV(CoV2-S), a lentivirus based pseudovirus expressing CoV2-S protein using HEK293T cells. We clone the single domain antibody (sdAb) sequence into a lentiviral vector (pLenti-GFP) for intracellular expression and assess not only the viral biogenesis but also the fate of the CoV2-S protein. Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.