CSIG-38. ENGINEERED PULLULAN-COLLAGEN-GOLD NANO COMPOSITE IMPROVES MESENCHYMAL STEM CELLS NEURAL DIFFERENTIATION AND INFLAMMATORY REGULATION

Tissue repair engineering supported by nanoparticles and stem cells is an efficient strategy for promoting the healing potential of damaged tissues. Tissue repair engineering supported by nanoparticles and stem cells is an efficient strategy for promoting the healing potential of damaged tissues. In this study, we prepared various nanomaterials including pure Pul, pure Col, Pul–Col, Pul–Au, Pul–Col–Au, and Col–Au to investigate their physicochemical properties, biocompatibility, biological functions, differentiation capacities, and anti-inflammatory abilities through in vitro and in vivo assessments. The physicochemical properties were characterized by SEM, DLS assay, contact angle measurements, UV-Vis spectra, FTIR spectra, SERS, and XPS analysis. Pul–Col–Au composite enhanced cell viability, promoted anti-oxidative ability for MSCs and HSFs, inhibited monocyte and platelet activation, induced the lowest cell apoptosis, facilitated the MMP activities and improved endothelialization. In conclusion, Pul–Col–Au nanoparticles are promising material for tissue regeneration.