Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022

Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tes...

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Veröffentlicht in:	Euro surveillance : bulletin européen sur les maladies transmissibles 2022-11, Vol.27 (45), p.1
Hauptverfasser:	Michel, Janine, Targosz, Angelina, Rinner, Thomas, Bourquain, Daniel, Brinkmann, Annika, Sacks, Jilian Amber, Schaade, Lars, Nitsche, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Berlin Deoxyribonucleic acid Diagnostic tests DNA DNA, Viral - analysis DNA, Viral - genetics Humans Medical diagnosis Monkeypox virus - genetics Mpox Mpox (monkeypox) - diagnosis Mpox (monkeypox) - epidemiology Polymerase Chain Reaction Rapid Communication Sensors
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Beschreibung
Zusammenfassung:	Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tested kits are currently intended for research use only and clinical performance still needs to be assessed in more detail, but all were suitable for diagnostics of monkeypox virus, with variations in specificity rather than sensitivity.
ISSN:	1560-7917 1025-496X 1560-7917
DOI:	10.2807/1560-7917.ES.2022.27.45.2200816