N-acetyltransferase 2 acetylator genotype-dependent N-acetylation and toxicity of the arylamine carcinogen β-naphthylamine in cryopreserved human hepatocytes

N-acetyltransferase 2 acetylator genotype-dependent N-acetylation and toxicity of the arylamine carcinogen β-naphthylamine in cryopreserved human hepatocytes

We used cryopreserved human hepatocytes that express rapid, intermediate, and slow acetylator N -acetyltransferase 2 (NAT2) genotypes to measure the N -acetylation of β-naphthylamine (BNA) which is one of the aromatic amines found in cigarette smoke including E-cigarettes. We investigated the role o...

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Veröffentlicht in:Archives of toxicology 2022-12, Vol.96 (12), p.3257-3263
Hauptverfasser: Habil, Mariam R., Salazar-González, Raúl A., Doll, Mark A., Hein, David W.
Format: Artikel
Sprache:eng
Schlagworte:
Acetylation
Acetyltransferase
Amines
Biomedical and Life Sciences
Biomedicine
Carcinogens
Cigarette smoke
Cigarettes
Cryopreservation
Damage
DNA damage
Environmental Health
Gene polymorphism
Genotoxicity
Genotypes
Hepatocytes
Incubation
N-Acetyltransferase 2
Occupational Medicine/Industrial Medicine
Organic compounds
Oxidative stress
Pharmacology/Toxicology
Polymorphism
Smoking
Toxicity
Toxicogenomics and Omics Technologies
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Zusammenfassung:We used cryopreserved human hepatocytes that express rapid, intermediate, and slow acetylator N -acetyltransferase 2 (NAT2) genotypes to measure the N -acetylation of β-naphthylamine (BNA) which is one of the aromatic amines found in cigarette smoke including E-cigarettes. We investigated the role of NAT2 genetic polymorphism in genotoxicity and oxidative stress induced by BNA. In vitro BNA NAT2 activities in rapid acetylators was 1.6 and 3.5-fold higher than intermediate ( p  
ISSN:0340-5761
1432-0738
DOI:10.1007/s00204-022-03381-4