SARS-CoV-2 genetic material is removed during municipal wastewater treatment and is undetectable after advanced treatment

The aim of this study was to establish whether SARS-CoV-2 genetic material is detectable after municipal wastewater treatment and to verify its expected removal from purified water that is reclaimed for potable reuse. Viral loads of SARS-CoV-2 (N1 and N2 genes) were monitored in raw influent wastewater (sewage) entering a water reclamation facility and in subsequent advanced treatment. Despite the large viral RNA load in raw sewage during peak COVID-19 outbreaks, substantial amounts of SARS-CoV-2 genetic material were removed during the conventional wastewater treatment process. Further, SARS-CoV-2 genetic material was undetectable after advanced purification. This confirms that potable reuse is resilient against high viral loads which are expected results given the advanced degree of wastewater and water treatment. Findings from this study may enhance public perception of the safety of potable water reuse; however, it should also be noted that studies to date worldwide indicate no evidence of SARS-CoV-2 transmission via water, and the CDC does not consider fecal waste or wastewaters as a source of exposure. [Display omitted] •First study to evaluate SARS-CoV-2 RNA throughout a potable reuse system.•SARS-CoV-2 RNA was rarely detected after conventional wastewater processes.•SARS-CoV-2 RNA was undetectable after advanced water treatment.•Potable reuse is an unlikely transmission route for COVID-19.•Water reuse practices are safe and effective, and protect public health.