Activation by gene amplification of pitB, encoding a third phosphate transporter of Escherichia coli K-12

Two systems for the uptake of inorganic phosphate (P(i)) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene am...

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Veröffentlicht in:Journal of bacteriology 2001-08, Vol.183 (15), p.4659-4663
Hauptverfasser: Hoffer, S M, Schoondermark, P, van Veen, H W, Tommassen, J
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container_title Journal of bacteriology
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creator Hoffer, S M
Schoondermark, P
van Veen, H W
Tommassen, J
description Two systems for the uptake of inorganic phosphate (P(i)) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene amplification event.
doi_str_mv 10.1128/jb.183.15.4659-4663.2001
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects ATP-Binding Cassette Transporters - genetics
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Carrier Proteins - genetics
Carrier Proteins - metabolism
DNA, Bacterial
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins
Gene Amplification
Genes
Genes, Bacterial
Genetics and Molecular Biology
Molecular Sequence Data
Mutagenesis
Periplasmic Binding Proteins
Phosphate-Binding Proteins
Phosphates
Phosphates - metabolism
title Activation by gene amplification of pitB, encoding a third phosphate transporter of Escherichia coli K-12
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