Activation by gene amplification of pitB, encoding a third phosphate transporter of Escherichia coli K-12

Two systems for the uptake of inorganic phosphate (P(i)) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene am...

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Veröffentlicht in:	Journal of bacteriology 2001-08, Vol.183 (15), p.4659-4663
Hauptverfasser:	Hoffer, S M, Schoondermark, P, van Veen, H W, Tommassen, J
Format:	Artikel
Sprache:	eng
Schlagworte:	ATP-Binding Cassette Transporters - genetics Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Carrier Proteins - genetics Carrier Proteins - metabolism DNA, Bacterial Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Gene Amplification Genes Genes, Bacterial Genetics and Molecular Biology Molecular Sequence Data Mutagenesis Periplasmic Binding Proteins Phosphate-Binding Proteins Phosphates Phosphates - metabolism
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Beschreibung
Zusammenfassung:	Two systems for the uptake of inorganic phosphate (P(i)) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on P(i) was isolated. We demonstrate that the expression of a new P(i) transporter, PitB, is activated in this strain by a gene amplification event.
ISSN:	0021-9193 1098-5530
DOI:	10.1128/jb.183.15.4659-4663.2001