Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos

The link between oxidative stress and environmental factors plays an important role in chronic degenerative diseases; therefore, exogenous antioxidants could be an effective alternative to combat disease progression and/or most significant symptoms. Curcuma longa L. (CL), commonly known as turmeric, is mostly composed of curcumin, a multivalent molecule described as having antioxidant, anti-inflammatory and neuroprotective properties. Poor chemical stability and low oral bioavailability and, consequently, poor absorption, rapid metabolism, and limited tissue distribution are major restrictions to its applicability. The advent of nanotechnology, by combining nanosacale with multi-functionality and bioavailability improvement, offers an opportunity to overcome these limitations. Therefore, in this work, poly-Ɛ-caprolactone (PCL) nanoparticles were developed to incorporate the methanolic extract of CL, and their bioactivity was assessed in comparison to free or encapsulated curcumin. Their toxicity was evaluated using zebrafish embryos by applying the Fish Embryo Acute Toxicity test, following recommended OECD guidelines. The protective effect against paraquat-induced oxidative damage of CL extract, free or encapsulated in PCL nanoparticles, was evaluated. This herbicide is known to cause oxidative damage and greatly affect neuromotor functions. The overall results indicate that CL-loaded PCL nanoparticles have an interesting protective capacity against paraquat-induced damage, particularly in neuromuscular development that goes well beyond that of CL extract itself and other known antioxidants. Marisa P. Sarria was supported by Marie Skłodowska—Curie Actions from European Union’s 7th Framework Programme for Research, Technological Development and Demonstration under Grant Agreement 600375. This work was supported by the strategic programme UID/BIA/04050/ 2019 funded by national funds through the Fundação para a Ciência e a Tecnologia I.P. Marisa P. Sárria was supported by Marie Curie COFUND funding from the European Union’s 7th Framework Programme for research, technological development, and demonstration under grant agreement 600375. Begoña Espiña is supported by the co-funding from Northern Regional Operational Program through the Project NORTE-45-2015-02 Nanotechnology based functional solutions. B.E. acknowledges the financial support of the project SbDtoolBox—Nanotechnology-based tools and tests for Safer-by-Design nanomaterials, with the reference nº N