260 The Effect of Different Bones and Analytical Methods on Assessment of Bone Mineralization Response to Dietary P, Phytase, and Vitamin D in Finishing Pigs

Pigs [n = 882; initial body weight (BW) of 33.2±0.31 kg] were used to evaluate the effects of different bones and analytical methods on assessment of bone mineralization response to dietary P, phytase, and vitamin D in growing-finishing pigs. Pens of pigs (20 pigs/pen) were completely randomized to 5 dietary treatments with 9 pens/treatment. After feeding diets for 112-d, nine pigs/treatment were harvested for bone analysis. Treatments were: 1) P at 80% of the NRC STTD P (deficient), 2) NRC STTD P using monocalcium phosphate, 3) NRC STTD P including phytase, 4) Industry P using monocalcium phosphate and phytase, 5) diet 4 with additional 2,000 IU/kg 25(OH)D3 (HyD). All diets contained vitamin D at 1,653 IU/kg. There were no significant differences for final BW, ADG, ADFI, G:F (P>0.10) or non-de-fat bone ash (bone ash×bone interaction, P>0.10). The response to treatment for bone density (Archimedes principle) and bone mineral content (DEXA) tended to depend on the bone (density×bone interaction, P=0.053; mineral density×bone interaction, P=0.157). There were no treatment differences (P>0.10) for bone density and bone mineral density between metacarpals, fibulas, and 2nd ribs. For 10th ribs, pigs fed industry levels of P had increased (P< 0.05) bone density compared with pigs fed NRC levels with phytase, with pigs fed deficient P, NRC P with no phytase, and excess vitamin D from HyD being intermediate. Pigs fed diets supplemented with HyD had increased bone mineral density in 10th ribs compared with pigs fed NRC levels of P with phytase, with pigs fed P deficient diets, industry P without HyD, and NRC P with monocalcium being intermediate. In summary, bone density and bone mineral content responses varied depending on the bone. Differences in bone density and mineral content in response to vitamin D and P were most apparent with the 10th ribs.