Comprehensive Analysis of Novel Genes and Pathways Associated with Osteogenic Differentiation of Adipose Stem Cells
Background. Adipose-derived stem cells (ADSCs) are an important alternative source of mesenchymal stem cells (MSCs) and show great promise in tissue engineering and regenerative medicine applications. However, identifying the novel genes and pathways and finding the underlying mechanisms regulating...
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| Veröffentlicht in: | Disease markers 2022-09, Vol.2022, p.1-11 |
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| creator | Gao, Qiuni Ma, Xiaorong Qi, Zuoliang |
| description | Background. Adipose-derived stem cells (ADSCs) are an important alternative source of mesenchymal stem cells (MSCs) and show great promise in tissue engineering and regenerative medicine applications. However, identifying the novel genes and pathways and finding the underlying mechanisms regulating ADSCs osteogenic differentiation remain urgent. Methods. We downloaded the gene expression profiles of GSE63754 and GSE37329 from the Gene Expression Omnibus (GEO) Database. We derived differentially expressed genes (DEGs) before and after ADSC osteogenic differentiation, followed by Gene Ontology (GO) functional and KEGG pathway analysis and protein-protein interaction (PPI) network analysis. 211 differentially expressed genes (142 upregulated genes and 69 downregulated genes) were aberrantly expressed. GO analysis revealed that these DEGs were associated with extracellular matrix organization, protein extracellular matrix, and semaphorin receptor binding. Conclusions. Our study provides novel genes and pathways that play important roles in regulating ADSC osteogenic differentiation, which may have potential therapeutic targets for clinic. |
| doi_str_mv | 10.1155/2022/4870981 |
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Adipose-derived stem cells (ADSCs) are an important alternative source of mesenchymal stem cells (MSCs) and show great promise in tissue engineering and regenerative medicine applications. However, identifying the novel genes and pathways and finding the underlying mechanisms regulating ADSCs osteogenic differentiation remain urgent. Methods. We downloaded the gene expression profiles of GSE63754 and GSE37329 from the Gene Expression Omnibus (GEO) Database. We derived differentially expressed genes (DEGs) before and after ADSC osteogenic differentiation, followed by Gene Ontology (GO) functional and KEGG pathway analysis and protein-protein interaction (PPI) network analysis. 211 differentially expressed genes (142 upregulated genes and 69 downregulated genes) were aberrantly expressed. GO analysis revealed that these DEGs were associated with extracellular matrix organization, protein extracellular matrix, and semaphorin receptor binding. Conclusions. Our study provides novel genes and pathways that play important roles in regulating ADSC osteogenic differentiation, which may have potential therapeutic targets for clinic.</description><identifier>ISSN: 0278-0240</identifier><identifier>EISSN: 1875-8630</identifier><identifier>DOI: 10.1155/2022/4870981</identifier><language>eng</language><publisher>Amsterdam: Hindawi</publisher><subject>Bioinformatics ; Bones ; Cell differentiation ; Cytokines ; Differentiation (biology) ; Extracellular matrix ; Fatty acids ; Gene expression ; Genes ; Genomics ; Lipoproteins ; Mesenchyme ; Metabolism ; Network analysis ; Ontology ; Protein interaction ; Proteins ; Regenerative medicine ; Software ; Stem cells ; Therapeutic targets ; Tissue engineering</subject><ispartof>Disease markers, 2022-09, Vol.2022, p.1-11</ispartof><rights>Copyright © 2022 Qiuni Gao et al.</rights><rights>Copyright © 2022 Qiuni Gao et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Qiuni Gao et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c382t-8062777324f421e2f5c2a3402b0358781d6b8c0dd85e61f7ed27d0beb54853e73</cites><orcidid>0000-0002-8730-065X ; 0000-0003-4656-3979</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484926/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484926/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><contributor>Shi, Jianxin</contributor><contributor>Jianxin Shi</contributor><creatorcontrib>Gao, Qiuni</creatorcontrib><creatorcontrib>Ma, Xiaorong</creatorcontrib><creatorcontrib>Qi, Zuoliang</creatorcontrib><title>Comprehensive Analysis of Novel Genes and Pathways Associated with Osteogenic Differentiation of Adipose Stem Cells</title><title>Disease markers</title><description>Background. Adipose-derived stem cells (ADSCs) are an important alternative source of mesenchymal stem cells (MSCs) and show great promise in tissue engineering and regenerative medicine applications. However, identifying the novel genes and pathways and finding the underlying mechanisms regulating ADSCs osteogenic differentiation remain urgent. Methods. We downloaded the gene expression profiles of GSE63754 and GSE37329 from the Gene Expression Omnibus (GEO) Database. We derived differentially expressed genes (DEGs) before and after ADSC osteogenic differentiation, followed by Gene Ontology (GO) functional and KEGG pathway analysis and protein-protein interaction (PPI) network analysis. 211 differentially expressed genes (142 upregulated genes and 69 downregulated genes) were aberrantly expressed. GO analysis revealed that these DEGs were associated with extracellular matrix organization, protein extracellular matrix, and semaphorin receptor binding. Conclusions. Our study provides novel genes and pathways that play important roles in regulating ADSC osteogenic differentiation, which may have potential therapeutic targets for clinic.</description><subject>Bioinformatics</subject><subject>Bones</subject><subject>Cell differentiation</subject><subject>Cytokines</subject><subject>Differentiation (biology)</subject><subject>Extracellular matrix</subject><subject>Fatty acids</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomics</subject><subject>Lipoproteins</subject><subject>Mesenchyme</subject><subject>Metabolism</subject><subject>Network analysis</subject><subject>Ontology</subject><subject>Protein interaction</subject><subject>Proteins</subject><subject>Regenerative medicine</subject><subject>Software</subject><subject>Stem cells</subject><subject>Therapeutic targets</subject><subject>Tissue engineering</subject><issn>0278-0240</issn><issn>1875-8630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><recordid>eNp9kU1rFEEQhhtRcI3e_AENXgQd05_TPRdh2WgihCRgcm56pmsyHWa616nZXfbfO5tdBD14qkM9PLxVLyHvOfvCudbngglxrqxhleUvyIJbowtbSvaSLJgwtmBCsdfkDeITY1xUqloQXOVhPUIHCeMW6DL5fo8RaW7pTd5CTy8hAVKfAr3zU7fze6RLxNxEP0Gguzh19BYnyI-QYkMvYtvCCGma1zGng2YZ4joj0J8TDHQFfY9vyavW9wjvTvOMPHz_dr-6Kq5vL3-sltdFI62YCstKYYyRQrVKcBCtboSXiomaSW2N5aGsbcNCsBpK3hoIwgRWQ62V1RKMPCNfj971ph4gNHOs0fduPcbBj3uXfXR_b1Ls3GPeukpZVYlyFnw8Ccb8awM4uSFiM5_gE-QNOmF4WUmllJ7RD_-gT3kzzt98powWQkk-U5-PVDNmxBHaP2E4c4cK3aFCd6pwxj8d8S6m4Hfx__RvzVScRw</recordid><startdate>20220912</startdate><enddate>20220912</enddate><creator>Gao, Qiuni</creator><creator>Ma, Xiaorong</creator><creator>Qi, Zuoliang</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8730-065X</orcidid><orcidid>https://orcid.org/0000-0003-4656-3979</orcidid></search><sort><creationdate>20220912</creationdate><title>Comprehensive Analysis of Novel Genes and Pathways Associated with Osteogenic Differentiation of Adipose Stem Cells</title><author>Gao, Qiuni ; Ma, Xiaorong ; Qi, Zuoliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-8062777324f421e2f5c2a3402b0358781d6b8c0dd85e61f7ed27d0beb54853e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bioinformatics</topic><topic>Bones</topic><topic>Cell differentiation</topic><topic>Cytokines</topic><topic>Differentiation (biology)</topic><topic>Extracellular matrix</topic><topic>Fatty acids</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomics</topic><topic>Lipoproteins</topic><topic>Mesenchyme</topic><topic>Metabolism</topic><topic>Network analysis</topic><topic>Ontology</topic><topic>Protein interaction</topic><topic>Proteins</topic><topic>Regenerative medicine</topic><topic>Software</topic><topic>Stem cells</topic><topic>Therapeutic targets</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Qiuni</creatorcontrib><creatorcontrib>Ma, Xiaorong</creatorcontrib><creatorcontrib>Qi, Zuoliang</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Qiuni</au><au>Ma, Xiaorong</au><au>Qi, Zuoliang</au><au>Shi, Jianxin</au><au>Jianxin Shi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive Analysis of Novel Genes and Pathways Associated with Osteogenic Differentiation of Adipose Stem Cells</atitle><jtitle>Disease markers</jtitle><date>2022-09-12</date><risdate>2022</risdate><volume>2022</volume><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>0278-0240</issn><eissn>1875-8630</eissn><abstract>Background. Adipose-derived stem cells (ADSCs) are an important alternative source of mesenchymal stem cells (MSCs) and show great promise in tissue engineering and regenerative medicine applications. However, identifying the novel genes and pathways and finding the underlying mechanisms regulating ADSCs osteogenic differentiation remain urgent. Methods. We downloaded the gene expression profiles of GSE63754 and GSE37329 from the Gene Expression Omnibus (GEO) Database. We derived differentially expressed genes (DEGs) before and after ADSC osteogenic differentiation, followed by Gene Ontology (GO) functional and KEGG pathway analysis and protein-protein interaction (PPI) network analysis. 211 differentially expressed genes (142 upregulated genes and 69 downregulated genes) were aberrantly expressed. GO analysis revealed that these DEGs were associated with extracellular matrix organization, protein extracellular matrix, and semaphorin receptor binding. Conclusions. Our study provides novel genes and pathways that play important roles in regulating ADSC osteogenic differentiation, which may have potential therapeutic targets for clinic.</abstract><cop>Amsterdam</cop><pub>Hindawi</pub><doi>10.1155/2022/4870981</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8730-065X</orcidid><orcidid>https://orcid.org/0000-0003-4656-3979</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bioinformatics Bones Cell differentiation Cytokines Differentiation (biology) Extracellular matrix Fatty acids Gene expression Genes Genomics Lipoproteins Mesenchyme Metabolism Network analysis Ontology Protein interaction Proteins Regenerative medicine Software Stem cells Therapeutic targets Tissue engineering |
| title | Comprehensive Analysis of Novel Genes and Pathways Associated with Osteogenic Differentiation of Adipose Stem Cells |
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