Potential Effects of Melatonin on TRPA1 Channels in the Prevention and Treatment of Alzheimer's Disease

Several studies have suggested that Melatonin (N-acetyl-5-methoxytryptamine) plays a protective role against many neurodegenerative events, including AD and Parkinson's disease (3,4). [...]decreased melatonin production in aged persons is considered a significant risk factor for developing AD. [...]we examined the role of melatonin on TRPA1 channels in neuroblastoma cells. Storage of cell lines for research does not require a HTA license. [...]the authors declare that there is no need for ethics committee approval and the study was carried out in accordance with Helsinki Declaration. Apoptosis Assay and Intracellular ROS Production Measurement APO Percentage™ (cell apoptosis assay) and the cell membrane penetration (by using non-charged and non-fluorescent dye [Dihydrorhodamine-123 (DHR-123)], was employed to determine and quantify apoptosis and cell membrane penetration according to our previous protocol (14,15).