The Agrobacterium tumefaciens chaperone-like protein, VirE1, interacts with VirE2 at domains required for single-stranded DNA binding and cooperative interaction

Agrobacterium tumefaciens transfers single-stranded DNA (ssDNA) into plants. Efficient tumorigenesis requires VirE1-dependent export of ssDNA-binding (SSB) protein VirE2. VirE1 binds VirE2 domains involved in SSB and self-association, and VirE1 may facilitate VirE2 export by preventing VirE2 aggrega...

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Veröffentlicht in:	Journal of bacteriology 1999-11, Vol.181 (21), p.6850-6855
Hauptverfasser:	Sundberg, C D, Ream, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens Agrobacterium tumefaciens - genetics Agrobacterium tumefaciens - metabolism Bacterial Proteins - metabolism Bacteriology Cloning, Molecular - methods Deoxyribonucleic acid DNA DNA, Single-Stranded - metabolism DNA-Binding Proteins - metabolism Ion Channels Molecular Chaperones - metabolism Plant Microbiology Plasmids - genetics Proteins
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Beschreibung
Zusammenfassung:	Agrobacterium tumefaciens transfers single-stranded DNA (ssDNA) into plants. Efficient tumorigenesis requires VirE1-dependent export of ssDNA-binding (SSB) protein VirE2. VirE1 binds VirE2 domains involved in SSB and self-association, and VirE1 may facilitate VirE2 export by preventing VirE2 aggregation and the premature binding of VirE2 to ssDNA.
ISSN:	0021-9193 1098-5530
DOI:	10.1128/jb.181.21.6850-6855.1999