Intravital longitudinal cellular visualization of oral mucosa in a murine model based on rotatory side-view confocal endomicroscopy
Oral mucosa is a soft tissue lining the inside of the mouth, protecting the oral cavity from microbiological insults. The mucosal immune system is composed of diverse types of cells that defend against a wide range of pathogens. The pathophysiology of various oral mucosal diseases has been studied m...
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Veröffentlicht in: | Biomedical optics express 2022-08, Vol.13 (8), p.4160-4174 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Oral mucosa is a soft tissue lining the inside of the mouth, protecting
the oral cavity from microbiological insults. The mucosal immune
system is composed of diverse types of cells that defend against a
wide range of pathogens. The pathophysiology of various oral mucosal
diseases has been studied mostly by ex vivo histological analysis of
harvested specimens. However, to analyze dynamic cellular processes in
the oral mucosa, longitudinal in vivo observation of the oral mucosa
in a single mouse during pathogenesis is a highly desirable and
efficient approach. Herein, by utilizing micro GRIN lens-based
rotatory side-view confocal endomicroscopy, we demonstrated
non-invasive longitudinal cellular-level in vivo imaging of the oral
mucosa, visualizing fluorescently labeled cells including various
immune cells, pericytes, nerve cells, and lymphatic and vascular
endothelial cells. With rotational and sliding movement of the
side-view endomicroscope on the oral mucosa, we successfully achieved
a multi-color wide-area cellular-level visualization in a noninvasive
manner. By using a transgenic mouse expressing photoconvertible
protein, Kaede, we achieved longitudinal repetitive imaging of the
same microscopic area in the buccal mucosa of a single mouse for up to
10 days. Finally, we performed longitudinal intravital visualization
of the oral mucosa in a DNFB-derived oral contact allergy mouse model,
which revealed highly dynamic spatiotemporal changes of CSF1R or LysM
expressing immune cells such as monocytes, macrophages, and
granulocytes in response to allergic challenge for one week. This
technique can be a useful tool to investigate the complex
pathophysiology of oral mucosal diseases. |
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ISSN: | 2156-7085 2156-7085 |
DOI: | 10.1364/BOE.462269 |