Sewage surveillance of SARS-CoV-2 at student campus residences in the Western Cape, South Africa

The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic capacity is limited in defined communities, posing a challenge in tracking and tracing new infections. Monitoring student residences, which are considered infection hotspots, with targeted wastewater surveillance is crucial. This study evaluated the efficacy of SARS-CoV-2 targeted wastewater surveillance for outbreak mitigation at Stellenbosch University's student residences in South Africa. Using torpedo-style passive sampling devices, wastewater samples were collected biweekly from manholes at twelve Stellenbosch University Tygerberg (SUT) campus and Stellenbosch University-Main (SUM) campus student residences. The surveillance led to an early warning detection of SARS-CoV-2 presence on campus, followed by an informed management strategy leading to restriction of student activities on campus and a delay in the onset of the third wave that was experienced throughout the country. Moreover, the study highlighted the extent of possible infections at defined locations even when a low number of confirmed coronavirus disease 2019 (COVID-19) cases were reported. The study also tracked the surge of the Delta and Omicron variants in the student residences using the Thermo Fisher TaqMan® RT-qPCR genotyping assay. [Display omitted] •Spatiotemporal targeted surveillance of SARS-CoV-2 viral RNA linked to student residences.•A steep rise in SARS-CoV-2 viral RNA was observed at the onset of the 4th wave.•The surveillance acted as an additional source of information to reduce student activities on campus.•Campus activity ceased, which coincided with reduced viral load.•Targeted surveillance proved effective for risk minimization and student activity decision-making.