Detection of synovial sepsis in horses using enzymes as biomarkers
Background Synovial sepsis is a commonly occurring, potentially career‐ending or even life‐threatening orthopaedic emergency. Diagnosis of synovial sepsis is currently primarily based on synovial fluid analysis, which often leaves diagnostic ambiguity due to overlap of clinicopathological parameters...
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Veröffentlicht in: | Equine veterinary journal 2022-05, Vol.54 (3), p.513-522 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Background
Synovial sepsis is a commonly occurring, potentially career‐ending or even life‐threatening orthopaedic emergency. Diagnosis of synovial sepsis is currently primarily based on synovial fluid analysis, which often leaves diagnostic ambiguity due to overlap of clinicopathological parameters between septic and aseptic inflammatory synovitis.
Objectives
To evaluate the reliability of lysozyme (LYS), myeloperoxidase (MPO) and elastase (ELT) as biomarkers for synovial sepsis in horses using a photometric assay to measure increased enzyme activity.
Study design
Prospective, single‐blinded, analytical, clinical study.
Methods
Equine synovial samples were assigned to one of three groups: (1) healthy controls (n = 10), (2) aseptic (n = 27) and (3) septic synovitis (n = 30). The enzyme activity assays (LYS, MPO and ELT) were compared with standard synovial fluid parameters and broad‐range bacterial 16S rDNA PCR.
Results
LYS and MPO activities were significantly different between septic synovial samples, and both aseptic and control samples (P |
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ISSN: | 0425-1644 2042-3306 |
DOI: | 10.1111/evj.13459 |