Mutations in the csgD promoter associated with variations in curli expression in certain strains of Escherichia coli O157:H7

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions....

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Veröffentlicht in:	Applied and environmental microbiology 2001-05, Vol.67 (5), p.2367-2370
Hauptverfasser:	UHLICH, Gaylen A, KEEN, James E, ELDER, Robert O
Format:	Artikel
Sprache:	eng
Schlagworte:	anatomy and morphology Bacteria Bacterial Proteins Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Biological and medical sciences cell structures csgD gene csgE gene csgF gene csgG gene dyes Escherichia coli Escherichia coli Infections Escherichia coli Infections - microbiology Escherichia coli O157 Escherichia coli O157 - genetics Escherichia coli O157 - growth & development Escherichia coli O157 - metabolism Escherichia coli Proteins Fundamental and applied biological sciences. Psychology genetic transformation Genetic Variation Genetics Genetics and Molecular Biology growth & development Humans intergenic DNA lacZ gene metabolism Microbiology Mutation phenotype plasmids promoter regions Promoter Regions, Genetic Promoter Regions, Genetic - genetics Sequence Analysis, DNA
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Zusammenfassung:	Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.67.5.2367-2370.2001