Rapid cryopreservation of small quantities of human spermatozoa by a self‐prepared cryoprotectant without animal component

Cryopreservation of small quantities of human spermatozoa whilst maintaining adequate post‐thawing motility has been found an essential challenge for male fertility preservation. Therefore, the study used an effective, and convenient rapid‐freezing method to freeze small amounts of human spermatozoa by adding self‐prepared cryoprotectant (SPC) without animal component. In the feasibility experiment, no significant differences in progressive motility, normal sperm morphology, vitality or DNA fragmentation index between the conventional slow freezing and rapid freezing were realised. The present study prospectively analysed the effects of sperm freezing and resuscitation in 175 patients with severe oligozoospermia (sperm concentration