Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and d...

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Veröffentlicht in:	Applied and environmental microbiology 2000-12, Vol.66 (12), p.5499-5502
Hauptverfasser:	LIHUA XIAO, LIMOR, Josef, MORGAN, U. N. A. M, SULAIMAN, Irshad M, THOMPSON, R. C, LAL, Altaf A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biodiversity Biological and medical sciences Cattle Classical genetics, quantitative genetics, hybrids COWP gene Cryptosporidium Cryptosporidium - genetics Disease DNA Primers - genetics DNA, Protozoan - genetics Dogs Environmental Microbiology and Biodegradation Fundamental and applied biological sciences. Psychology Genes Genes, Protozoan Genetics of eukaryotes. Biological and molecular evolution Humans Mice Molecular biology Molecular Sequence Data oocyst wall protein Parasites Polymerase Chain Reaction Proteins Protozoa Protozoan Proteins - genetics Sequence Homology, Nucleic Acid Species Specificity
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Zusammenfassung:	Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.66.12.5499-5502.2000