Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies

Drug-inducible suicide systems may help to minimize risks of human induced pluripotent stem cell (hiPSC) therapies. Recent research challenged the usefulness of such systems since rare drug-resistant subclones were observed. We have introduced a drug-inducible Caspase 9 suicide system (iCASP9) into...

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Veröffentlicht in:Molecular therapy. Methods & clinical development 2022-09, Vol.26, p.84-94
Hauptverfasser: Wunderlich, Stephanie, Haase, Alexandra, Merkert, Sylvia, Jahn, Kirsten, Deest, Maximillian, Frieling, Helge, Glage, Silke, Korte, Wilhelm, Martens, Andreas, Kirschning, Andreas, Zeug, Andre, Ponimaskin, Evgeni, Göhring, Gudrun, Ackermann, Mania, Lachmann, Nico, Moritz, Thomas, Zweigerdt, Robert, Martin, Ulrich
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Sprache:eng
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Zusammenfassung:Drug-inducible suicide systems may help to minimize risks of human induced pluripotent stem cell (hiPSC) therapies. Recent research challenged the usefulness of such systems since rare drug-resistant subclones were observed. We have introduced a drug-inducible Caspase 9 suicide system (iCASP9) into the AAVS1 safe-harbor locus of hiPSCs. In these cells, apoptosis could be efficiently induced in vitro. After transplantation into mice, drug treatment generally led to rapid elimination of teratomas, but single animals subsequently formed tumor tissue from monoallelic iCASP9 hiPSCs. Very rare drug-resistant subclones of monoallelic iCASP9 hiPSCs appeared in vitro with frequencies of ∼ 3 × 10-8. Besides transgene elimination, presumably via loss of heterozygosity (LoH), silencing via aberrant promoter methylation was identified as a major underlying mechanism. In contrast to monoallelic iCASP9 hiPSCs, no escapees from biallelic iCASP9 cells were observed after treatment of up to 0.8 billion hiPSCs. The highly increased safety level provided by biallelic integration of the iCASP9 system may substantially contribute to the safety level of iPSC-based therapies. [Display omitted] Drug-inducible suicide systems may help to minimize risks of hiPSC therapies. Monoallelic integration of a Caspase 9 system allowed formation of drug-resistant iPSC clones due to loss of heterozygosity or promoter methylation. In contrast, no escapees were observed after treatment of high numbers of cells with biallelic integration of the Caspase9 system.
ISSN:2329-0501
2329-0501
DOI:10.1016/j.omtm.2022.05.011