Imaging of innate immunity activation in vivo with a redox-tuned PET reporter
High-redox-potential reactive oxygen species and reactive nitrogen species (ROS/RNS), generated by NADPH oxidase-2 (NOX2), myeloperoxidase (MPO) and related enzymes, are key effector molecules of innate immunity. High-redox-potential radicals are difficult to distinguish by imaging from less potent...
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Veröffentlicht in: | Nature biotechnology 2022-06, Vol.40 (6), p.965-973 |
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Sprache: | eng |
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Zusammenfassung: | High-redox-potential reactive oxygen species and reactive nitrogen species (ROS/RNS), generated by NADPH oxidase-2 (NOX2), myeloperoxidase (MPO) and related enzymes, are key effector molecules of innate immunity. High-redox-potential radicals are difficult to distinguish by imaging from less potent ROS/RNS functioning as background biological signaling molecules. Here we present 4-[
18
F]fluoro-1-naphthol ([
18
F]4FN), a redox-tuned radiopharmaceutical that selectively binds proteins and cells when oxidized by products of human MPO plus H
2
O
2
, but not H
2
O
2
alone, and can be detected using positron emission tomography (PET). Activating HL-60 neutrophil-like human cells with phorbol ester (PMA) caused [
18
F]4FN retention five-fold over unstimulated cells. An MPO-specific inhibitor (4-ABAH) blocked cellular retention by more than 95%. [
18
F]4FN PET/CT imaging discriminated inflammatory foci in vivo in three murine models of activated innate immunity: endotoxin-induced toxic shock, PMA-induced contact dermatitis and lipopolysaccharide-induced ankle arthritis. 4-ABAH and Cybb
−/−
(Nox2
−/−
) gene deletion strongly abrogated [
18
F]4FN retention in vivo. Thus, [
18
F]4FN shows promise as a robust reporter of innate immunity activation by PET/CT.
Inflammation is imaged in mice with a PET reporter of high-energy reactive oxygen and nitrogen species. |
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ISSN: | 1087-0156 1546-1696 |
DOI: | 10.1038/s41587-021-01169-y |