Case Study: Room Fumigation Using Aerosolized Hydrogen Peroxide—A Versatile and Economic Fumigation Method

Introduction: Formaldehyde is still the method of choice for fumigation of rooms and HEPA filters at high- and maximum-containment facilities because of its proven track record and low cost. However, formaldehyde has been shown to be carcinogenic and should ideally be replaced by other, less hazardous methods. This change has in part been hampered by the relatively high cost of alternative methods. Methods: Here, we provide examples of room fumigations using aerosolized hydrogen peroxide showing not only that it can be used economically but also that it is a versatile method and may be used under circumstances not normally suited for fumigation. Results and Discussion: Four examples of fumigation setups are presented that illustrate the versatility, ease of use, and adaptability of aerosolized hydrogen peroxide as a fumigant. In addition, we demonstrate that aerosolized hydrogen peroxide passes through HEPA filters in biological safety cabinets and individually ventilated cage racks. Conclusions: Considering that the fumigation method presented here is simple and highly effective, we expect it to serve as a relatively cost-effective alternative to formaldehyde fumigation for disinfecting potentially contaminated rooms and surfaces.