Expansion Microscopy on Saccharomyces cerevisiae
The unicellular eukaryote S. cerevisiae represents an invaluable resource for the study of basic eukaryotic cellular and molecular processes. The combination of a high genetic amenability, numerous genetic tools, and vast genomic resources makes it one of the most versatile model organisms, employed...
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Veröffentlicht in: | microPublication biology 2022, Vol.2022 |
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Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The unicellular eukaryote S. cerevisiae represents an invaluable resource for the study of basic eukaryotic cellular and molecular processes. The combination of a high genetic amenability, numerous genetic tools, and vast genomic resources makes it one of the most versatile model organisms, employed in a wide range of basic research disciplines. However, its small size compared to other eukaryotic organisms has limited its use for the study of sub-cellular structures. The diameter of unbudded yeast cells ranges approximately between 4 μm in haploids and 6 μm in diploids (Milo and Phillips 2015), complicating conventional diffraction-limited light microscopy approaches. |
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ISSN: | 2578-9430 |
DOI: | 10.17912/micropub.biology.000566 |