Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry

Rapid, direct methods are needed to assess active bacterial populations in water and foods. Our objective was to determine the efficiency of bacterial detection by immunomagnetic separation (IMS) and the compatibility of IMS with cyanoditolyl tetrazolium chloride (CTC) incubation to determine respir...

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Veröffentlicht in:	Applied and environmental microbiology 1999-05, Vol.65 (5), p.1966-1972
Hauptverfasser:	Pyle, B. H., Broadaway, S. C., McFeters, G. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria Bacteriological Techniques - statistics & numerical data Biological and medical sciences Cattle Escherichia coli Escherichia coli O157 - isolation & purification Fluorescent Dyes Food Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Immunomagnetic Separation - methods Lasers Life Sciences (General) Meat - microbiology Microbiology Microscopes Microscopy, Fluorescence Regression Analysis Tetrazolium Salts Water Water Microbiology
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Beschreibung
Zusammenfassung:	Rapid, direct methods are needed to assess active bacterial populations in water and foods. Our objective was to determine the efficiency of bacterial detection by immunomagnetic separation (IMS) and the compatibility of IMS with cyanoditolyl tetrazolium chloride (CTC) incubation to determine respiratory activity, using the pathogen Escherichia coli O157:H7. Counterstaining with a specific fluorescein-conjugated anti-O157 antibody (FAb) following CTC incubation was used to allow confirmation and visualization of bacteria by epifluorescence microscopy. Broth-grown E. coli O157:H7 was used to inoculate fresh ground beef (
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.65.5.1966-1972.1999