Molecular Monitoring of SARS-CoV‑2 in Different Sewage Plants in Venice and the Implications for Genetic Surveillance

Wastewater-based epidemiology is now widely used as an indirect tool to monitor the spread of SARS-CoV-2. In this study, five different sample matrices representing diverse phases of the wastewater treatment process were collected during the second wave of SARS-CoV-2 from two wastewater treatment pl...

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Veröffentlicht in:ACS ES&T water 2022-11, Vol.2 (11), p.1953-1963
Hauptverfasser: Brian, Irene, Manuzzi, Alice, Dalla Rovere, Giulia, Giussani, Edoardo, Palumbo, Elisa, Fusaro, Alice, Bonfante, Francesco, Bortolami, Alessio, Quaranta, Erika Giorgia, Monne, Isabella, Patarnello, Tomaso, Bargelloni, Luca, Terregino, Calogero, Holmes, Edward C., Todesco, Giovanna, Sorrentino, Francesco, Berton, Andrea, Badetti, Christian, Carrer, Claudio, Ferrari, Giorgio, Zincone, Cinzia, Milan, Massimo, Panzarin, Valentina
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Sprache:eng
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Zusammenfassung:Wastewater-based epidemiology is now widely used as an indirect tool to monitor the spread of SARS-CoV-2. In this study, five different sample matrices representing diverse phases of the wastewater treatment process were collected during the second wave of SARS-CoV-2 from two wastewater treatment plants (WWTPs) serving the Civil Hospital and Sacca Fisola island in Venice, Italy. Positive SARS-CoV-2 detections occurred at both WWTPs, and data on viral genome detection rate and quantification suggest that the pellet (i.e., the particulate resulting from the influent) is a sensitive matrix that permits reliable assessment of infection prevalence while reducing time to results. On the contrary, analysis of post-treatment matrices provides evidence of the decontamination efficacy of both WWTPs. Finally, direct sequencing of wastewater samples enabled us to identify B.1.177 and B.1.160 as the prevalent SARS-CoV-2 lineages circulating in Venice at the time of sampling. This study confirmed the suitability of wastewater testing for studying SARS-CoV-2 circulation and established a simplified workflow for the prompt detection and characterization of the virus.
ISSN:2690-0637
2690-0637
DOI:10.1021/acsestwater.2c00013