CRISPR–Cas12-based detection of SARS-CoV-2
An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (
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| Veröffentlicht in: | Nature biotechnology 2020-07, Vol.38 (7), p.870-874 |
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| container_title | Nature biotechnology |
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| creator | Broughton, James P. Deng, Xianding Yu, Guixia Fasching, Clare L. Servellita, Venice Singh, Jasmeet Miao, Xin Streithorst, Jessica A. Granados, Andrea Sotomayor-Gonzalez, Alicia Zorn, Kelsey Gopez, Allan Hsu, Elaine Gu, Wei Miller, Steve Pan, Chao-Yang Guevara, Hugo Wadford, Debra A. Chen, Janice S. Chiu, Charles Y. |
| description | An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid ( |
| doi_str_mv | 10.1038/s41587-020-0513-4 |
| format | Article |
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SARS-CoV-2 in patient samples is detected in under an hour using a CRISPR-based lateral flow assay.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/s41587-020-0513-4</identifier><identifier>PMID: 32300245</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/326/107 ; 631/326/2521 ; 631/326/596/4130 ; 692/699/255 ; 692/699/255/2514 ; Agriculture ; Assaying ; Betacoronavirus - genetics ; Betacoronavirus - isolation & purification ; Biochemical assays ; Bioinformatics ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Clinical Laboratory Techniques ; Coronavirus Infections - diagnosis ; Coronavirus Infections - virology ; Coronaviruses ; COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; CRISPR ; CRISPR-Cas Systems ; Disease control ; Genetic aspects ; Genetic engineering ; Health aspects ; Humans ; Letter ; Life Sciences ; Methods ; Nucleic Acid Amplification Techniques - methods ; Pandemics ; Pneumonia, Viral - diagnosis ; Pneumonia, Viral - virology ; Respiratory diseases ; Ribonucleic acid ; RNA ; RNA, Guide, CRISPR-Cas Systems - genetics ; SARS-CoV-2 ; Severe acute respiratory syndrome coronavirus 2 ; Time Factors ; Viral diseases</subject><ispartof>Nature biotechnology, 2020-07, Vol.38 (7), p.870-874</ispartof><rights>The Author(s), under exclusive licence to Springer Nature America, Inc. 2020</rights><rights>COPYRIGHT 2020 Nature Publishing Group</rights><rights>The Author(s), under exclusive licence to Springer Nature America, Inc. 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c698t-e7211fbece5a2f1fcc89f0231a99910b552fd414d813a7d820f0192039894ce53</citedby><cites>FETCH-LOGICAL-c698t-e7211fbece5a2f1fcc89f0231a99910b552fd414d813a7d820f0192039894ce53</cites><orcidid>0000-0001-5852-9546 ; 0000-0003-4797-3056 ; 0000-0003-2915-2094</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32300245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Broughton, James P.</creatorcontrib><creatorcontrib>Deng, Xianding</creatorcontrib><creatorcontrib>Yu, Guixia</creatorcontrib><creatorcontrib>Fasching, Clare L.</creatorcontrib><creatorcontrib>Servellita, Venice</creatorcontrib><creatorcontrib>Singh, Jasmeet</creatorcontrib><creatorcontrib>Miao, Xin</creatorcontrib><creatorcontrib>Streithorst, Jessica A.</creatorcontrib><creatorcontrib>Granados, Andrea</creatorcontrib><creatorcontrib>Sotomayor-Gonzalez, Alicia</creatorcontrib><creatorcontrib>Zorn, Kelsey</creatorcontrib><creatorcontrib>Gopez, Allan</creatorcontrib><creatorcontrib>Hsu, Elaine</creatorcontrib><creatorcontrib>Gu, Wei</creatorcontrib><creatorcontrib>Miller, Steve</creatorcontrib><creatorcontrib>Pan, Chao-Yang</creatorcontrib><creatorcontrib>Guevara, Hugo</creatorcontrib><creatorcontrib>Wadford, Debra A.</creatorcontrib><creatorcontrib>Chen, Janice S.</creatorcontrib><creatorcontrib>Chiu, Charles Y.</creatorcontrib><title>CRISPR–Cas12-based detection of SARS-CoV-2</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
SARS-CoV-2 in patient samples is detected in under an hour using a CRISPR-based lateral flow assay.</description><subject>631/326/107</subject><subject>631/326/2521</subject><subject>631/326/596/4130</subject><subject>692/699/255</subject><subject>692/699/255/2514</subject><subject>Agriculture</subject><subject>Assaying</subject><subject>Betacoronavirus - genetics</subject><subject>Betacoronavirus - isolation & purification</subject><subject>Biochemical assays</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Clinical Laboratory Techniques</subject><subject>Coronavirus Infections - diagnosis</subject><subject>Coronavirus Infections - virology</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>COVID-19 Testing</subject><subject>COVID-19 Vaccines</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems</subject><subject>Disease control</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Letter</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Nucleic Acid Amplification Techniques - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Broughton, James P.</au><au>Deng, Xianding</au><au>Yu, Guixia</au><au>Fasching, Clare L.</au><au>Servellita, Venice</au><au>Singh, Jasmeet</au><au>Miao, Xin</au><au>Streithorst, Jessica A.</au><au>Granados, Andrea</au><au>Sotomayor-Gonzalez, Alicia</au><au>Zorn, Kelsey</au><au>Gopez, Allan</au><au>Hsu, Elaine</au><au>Gu, Wei</au><au>Miller, Steve</au><au>Pan, Chao-Yang</au><au>Guevara, Hugo</au><au>Wadford, Debra A.</au><au>Chen, Janice S.</au><au>Chiu, Charles Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CRISPR–Cas12-based detection of SARS-CoV-2</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>38</volume><issue>7</issue><spage>870</spage><epage>874</epage><pages>870-874</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><abstract>An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
SARS-CoV-2 in patient samples is detected in under an hour using a CRISPR-based lateral flow assay.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>32300245</pmid><doi>10.1038/s41587-020-0513-4</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-5852-9546</orcidid><orcidid>https://orcid.org/0000-0003-4797-3056</orcidid><orcidid>https://orcid.org/0000-0003-2915-2094</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-0156 |
| ispartof | Nature biotechnology, 2020-07, Vol.38 (7), p.870-874 |
| issn | 1087-0156 1546-1696 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9107629 |
| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | 631/326/107 631/326/2521 631/326/596/4130 692/699/255 692/699/255/2514 Agriculture Assaying Betacoronavirus - genetics Betacoronavirus - isolation & purification Biochemical assays Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Clinical Laboratory Techniques Coronavirus Infections - diagnosis Coronavirus Infections - virology Coronaviruses COVID-19 COVID-19 Testing COVID-19 Vaccines CRISPR CRISPR-Cas Systems Disease control Genetic aspects Genetic engineering Health aspects Humans Letter Life Sciences Methods Nucleic Acid Amplification Techniques - methods Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Respiratory diseases Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Time Factors Viral diseases |
| title | CRISPR–Cas12-based detection of SARS-CoV-2 |
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