CRISPR–Cas12-based detection of SARS-CoV-2

An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (

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Veröffentlicht in:	Nature biotechnology 2020-07, Vol.38 (7), p.870-874
Hauptverfasser:	Broughton, James P., Deng, Xianding, Yu, Guixia, Fasching, Clare L., Servellita, Venice, Singh, Jasmeet, Miao, Xin, Streithorst, Jessica A., Granados, Andrea, Sotomayor-Gonzalez, Alicia, Zorn, Kelsey, Gopez, Allan, Hsu, Elaine, Gu, Wei, Miller, Steve, Pan, Chao-Yang, Guevara, Hugo, Wadford, Debra A., Chen, Janice S., Chiu, Charles Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/326/107 631/326/2521 631/326/596/4130 692/699/255 692/699/255/2514 Agriculture Assaying Betacoronavirus - genetics Betacoronavirus - isolation & purification Biochemical assays Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Clinical Laboratory Techniques Coronavirus Infections - diagnosis Coronavirus Infections - virology Coronaviruses COVID-19 COVID-19 Testing COVID-19 Vaccines CRISPR CRISPR-Cas Systems Disease control Genetic aspects Genetic engineering Health aspects Humans Letter Life Sciences Methods Nucleic Acid Amplification Techniques - methods Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Respiratory diseases Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Time Factors Viral diseases
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Zusammenfassung:	An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (
ISSN:	1087-0156 1546-1696
DOI:	10.1038/s41587-020-0513-4