Study on the identification of Pinelliae rhizoma and Pinelliae pedatisectae rhizoma based on the characteristic component triglochinic acid

For the first time, a monomeric compound, triglochinic acid, has been isolated from the tubers of Schott with its structure analyzed using NMR. Its molecular formula is C H O , with the chemical name (2 )-but-2-ene-1,2,4-tricarboxylic acid. Through HPLC-DAD and HPLC-MS analysis studies, it has concluded that does not contain triglochinic acid, while does. This key observation was used as a characteristic component to distinguish these two herbs. We analyzed 39 batches of collected in herbal medicine market, among which triglochinic acid was detected in 27 batches, resulting in a adulteration ratio of reaching 69.2%. Our method demonstrates great potential for authenticating the products, thus ensuring the quality of .