Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film
Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts. However, the recognition and separation of specific bacteria in complex matrices are still challenging. Herein, a bacteria-imprinted polydimethylsiloxane (PDMS) film was f...
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| Veröffentlicht in: | RSC advances 2020-04, Vol.10 (24), p.14305-14312 |
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| creator | Fu, Kaiyue Zhang, Huiwen Guo, Yuanyuan Li, Juan Nie, Heran Song, Xiuling Xu, Kun Wang, Juan Zhao, Chao |
| description | Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts. However, the recognition and separation of specific bacteria in complex matrices are still challenging. Herein, a bacteria-imprinted polydimethylsiloxane (PDMS) film was facilely prepared and modified with 1
,1
,2
,2
-perfluorooctyltriethoxysilane (POTS). Employing
as a model bacterium, the imprinted surface exhibited three-dimensionality cavities with mean size of 1000 × 800 nm in square and 100 nm in depth. After incubation for 2 h with 6 × 10
CFU mL
of
, the imprinted polymer film can reach a 62.9% capture efficiency. Furthermore, the imprinted POTS-modified PDMS film based solid phase extraction combined with polymerase chain reaction and agarose gel electrophoresis allows for detecting 10
CFU mL
with excellent selectivity in fresh oyster samples. As a result, the developed selective sample pretreatment method using molecular imprinting technology provides a promising platform for separation, identification, and analysis of pathogens. |
| doi_str_mv | 10.1039/d0ra00306a |
| format | Article |
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,1
,2
,2
-perfluorooctyltriethoxysilane (POTS). Employing
as a model bacterium, the imprinted surface exhibited three-dimensionality cavities with mean size of 1000 × 800 nm in square and 100 nm in depth. After incubation for 2 h with 6 × 10
CFU mL
of
, the imprinted polymer film can reach a 62.9% capture efficiency. Furthermore, the imprinted POTS-modified PDMS film based solid phase extraction combined with polymerase chain reaction and agarose gel electrophoresis allows for detecting 10
CFU mL
with excellent selectivity in fresh oyster samples. As a result, the developed selective sample pretreatment method using molecular imprinting technology provides a promising platform for separation, identification, and analysis of pathogens.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d0ra00306a</identifier><identifier>PMID: 35498485</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Alkoxysilanes ; Bacteria ; Chemistry ; Electrophoresis ; Imprinted polymers ; Molecular imprinting ; Polydimethylsiloxane ; Polymer films ; Polymerase chain reaction ; Pretreatment ; Recognition ; Selectivity ; Separation ; Silicone resins ; Solid phases</subject><ispartof>RSC advances, 2020-04, Vol.10 (24), p.14305-14312</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2020</rights><rights>This journal is © The Royal Society of Chemistry 2020 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-cac6117b1345bdf83b7bfdf38f6a3351da3b7214f142a136d1dc214b1fa1dd523</citedby><cites>FETCH-LOGICAL-c406t-cac6117b1345bdf83b7bfdf38f6a3351da3b7214f142a136d1dc214b1fa1dd523</cites><orcidid>0000-0003-2773-789X ; 0000-0003-1796-2284 ; 0000-0003-1309-9444 ; 0000-0002-2742-2743 ; 0000-0003-3569-2732</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051946/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051946/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35498485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Kaiyue</creatorcontrib><creatorcontrib>Zhang, Huiwen</creatorcontrib><creatorcontrib>Guo, Yuanyuan</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Nie, Heran</creatorcontrib><creatorcontrib>Song, Xiuling</creatorcontrib><creatorcontrib>Xu, Kun</creatorcontrib><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Zhao, Chao</creatorcontrib><title>Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts. However, the recognition and separation of specific bacteria in complex matrices are still challenging. Herein, a bacteria-imprinted polydimethylsiloxane (PDMS) film was facilely prepared and modified with 1
,1
,2
,2
-perfluorooctyltriethoxysilane (POTS). Employing
as a model bacterium, the imprinted surface exhibited three-dimensionality cavities with mean size of 1000 × 800 nm in square and 100 nm in depth. After incubation for 2 h with 6 × 10
CFU mL
of
, the imprinted polymer film can reach a 62.9% capture efficiency. Furthermore, the imprinted POTS-modified PDMS film based solid phase extraction combined with polymerase chain reaction and agarose gel electrophoresis allows for detecting 10
CFU mL
with excellent selectivity in fresh oyster samples. As a result, the developed selective sample pretreatment method using molecular imprinting technology provides a promising platform for separation, identification, and analysis of pathogens.</description><subject>Alkoxysilanes</subject><subject>Bacteria</subject><subject>Chemistry</subject><subject>Electrophoresis</subject><subject>Imprinted polymers</subject><subject>Molecular imprinting</subject><subject>Polydimethylsiloxane</subject><subject>Polymer films</subject><subject>Polymerase chain reaction</subject><subject>Pretreatment</subject><subject>Recognition</subject><subject>Selectivity</subject><subject>Separation</subject><subject>Silicone resins</subject><subject>Solid phases</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkc1qFjEYhYMoband9AIk4EaEzyaTn2Y2wketWigUiroN7-SnTc1MxmSmdG7CazZja6nNJsnJk8N5OQgdUvKBEtYeWZKBEEYkvEB7DeFy0xDZvnxy3kUHpdyQuqSgjaQ7aJcJ3iquxB76fQljsBgGi4uLzkzh1uHsTLoawhTSgJPHP0KXQ8IjZLgG16e4TMHMBUMpoUzO4m7Bo8s-zimHAVYF4s90t5QQYXC4Tzb4UNX61Zk5Qo4LDv1Y4ZUdq2HvMvYh9q_RKw-xuIOHfR99_3z67eTr5vziy9nJ9nxjOJHTxoCRlB53lHHRWa9Yd9x565nyEhgT1EJVGso95Q1QJi21pl476oFaKxq2jz7e-45z1ztr3DBliLpG6iEvOkHQ_78M4VpfpVvdEkFbLqvBuweDnH7Nrky6D8W4uA6c5qIbKZTkQkhV0bfP0Js056GOpxumWtEqqdZE7-8pk1Mp2fnHMJTotWn9iVxu_za9rfCbp_Ef0X-9sj8d6Kja</recordid><startdate>20200407</startdate><enddate>20200407</enddate><creator>Fu, Kaiyue</creator><creator>Zhang, Huiwen</creator><creator>Guo, Yuanyuan</creator><creator>Li, Juan</creator><creator>Nie, Heran</creator><creator>Song, Xiuling</creator><creator>Xu, Kun</creator><creator>Wang, Juan</creator><creator>Zhao, Chao</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2773-789X</orcidid><orcidid>https://orcid.org/0000-0003-1796-2284</orcidid><orcidid>https://orcid.org/0000-0003-1309-9444</orcidid><orcidid>https://orcid.org/0000-0002-2742-2743</orcidid><orcidid>https://orcid.org/0000-0003-3569-2732</orcidid></search><sort><creationdate>20200407</creationdate><title>Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film</title><author>Fu, Kaiyue ; Zhang, Huiwen ; Guo, Yuanyuan ; Li, Juan ; Nie, Heran ; Song, Xiuling ; Xu, Kun ; Wang, Juan ; Zhao, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-cac6117b1345bdf83b7bfdf38f6a3351da3b7214f142a136d1dc214b1fa1dd523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkoxysilanes</topic><topic>Bacteria</topic><topic>Chemistry</topic><topic>Electrophoresis</topic><topic>Imprinted polymers</topic><topic>Molecular imprinting</topic><topic>Polydimethylsiloxane</topic><topic>Polymer films</topic><topic>Polymerase chain reaction</topic><topic>Pretreatment</topic><topic>Recognition</topic><topic>Selectivity</topic><topic>Separation</topic><topic>Silicone resins</topic><topic>Solid phases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Kaiyue</creatorcontrib><creatorcontrib>Zhang, Huiwen</creatorcontrib><creatorcontrib>Guo, Yuanyuan</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Nie, Heran</creatorcontrib><creatorcontrib>Song, Xiuling</creatorcontrib><creatorcontrib>Xu, Kun</creatorcontrib><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Zhao, Chao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Kaiyue</au><au>Zhang, Huiwen</au><au>Guo, Yuanyuan</au><au>Li, Juan</au><au>Nie, Heran</au><au>Song, Xiuling</au><au>Xu, Kun</au><au>Wang, Juan</au><au>Zhao, Chao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2020-04-07</date><risdate>2020</risdate><volume>10</volume><issue>24</issue><spage>14305</spage><epage>14312</epage><pages>14305-14312</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts. However, the recognition and separation of specific bacteria in complex matrices are still challenging. Herein, a bacteria-imprinted polydimethylsiloxane (PDMS) film was facilely prepared and modified with 1
,1
,2
,2
-perfluorooctyltriethoxysilane (POTS). Employing
as a model bacterium, the imprinted surface exhibited three-dimensionality cavities with mean size of 1000 × 800 nm in square and 100 nm in depth. After incubation for 2 h with 6 × 10
CFU mL
of
, the imprinted polymer film can reach a 62.9% capture efficiency. Furthermore, the imprinted POTS-modified PDMS film based solid phase extraction combined with polymerase chain reaction and agarose gel electrophoresis allows for detecting 10
CFU mL
with excellent selectivity in fresh oyster samples. As a result, the developed selective sample pretreatment method using molecular imprinting technology provides a promising platform for separation, identification, and analysis of pathogens.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35498485</pmid><doi>10.1039/d0ra00306a</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2773-789X</orcidid><orcidid>https://orcid.org/0000-0003-1796-2284</orcidid><orcidid>https://orcid.org/0000-0003-1309-9444</orcidid><orcidid>https://orcid.org/0000-0002-2742-2743</orcidid><orcidid>https://orcid.org/0000-0003-3569-2732</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Alkoxysilanes Bacteria Chemistry Electrophoresis Imprinted polymers Molecular imprinting Polydimethylsiloxane Polymer films Polymerase chain reaction Pretreatment Recognition Selectivity Separation Silicone resins Solid phases |
| title | Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film |
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