Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film
Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts. However, the recognition and separation of specific bacteria in complex matrices are still challenging. Herein, a bacteria-imprinted polydimethylsiloxane (PDMS) film was f...
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Veröffentlicht in: | RSC advances 2020-04, Vol.10 (24), p.14305-14312 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts. However, the recognition and separation of specific bacteria in complex matrices are still challenging. Herein, a bacteria-imprinted polydimethylsiloxane (PDMS) film was facilely prepared and modified with 1
,1
,2
,2
-perfluorooctyltriethoxysilane (POTS). Employing
as a model bacterium, the imprinted surface exhibited three-dimensionality cavities with mean size of 1000 × 800 nm in square and 100 nm in depth. After incubation for 2 h with 6 × 10
CFU mL
of
, the imprinted polymer film can reach a 62.9% capture efficiency. Furthermore, the imprinted POTS-modified PDMS film based solid phase extraction combined with polymerase chain reaction and agarose gel electrophoresis allows for detecting 10
CFU mL
with excellent selectivity in fresh oyster samples. As a result, the developed selective sample pretreatment method using molecular imprinting technology provides a promising platform for separation, identification, and analysis of pathogens. |
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ISSN: | 2046-2069 2046-2069 |
DOI: | 10.1039/d0ra00306a |