Diamond Raman laser and Yb fiber amplifier for in vivo multiphoton fluorescence microscopy

Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for two-photon excitation of red-shifted fluorophores. These custom excitation sources are both simple to construct and cost-efficient in comparison to simi...

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Veröffentlicht in:Biomedical optics express 2022-04, Vol.13 (4), p.1888-1898
Hauptverfasser: Engelmann, Shaun A, Zhou, Annie, Hassan, Ahmed M, Williamson, Michael R, Jarrett, Jeremy W, Perillo, Evan P, Tomar, Alankrit, Spence, David J, Jones, Theresa A, Dunn, Andrew K
Format: Artikel
Sprache:eng
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Zusammenfassung:Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for two-photon excitation of red-shifted fluorophores. These custom excitation sources are both simple to construct and cost-efficient in comparison to similar custom and commercial alternatives. Furthermore, they operate at a repetition rate (80 MHz) that allows fast image acquisition using resonant scanners. With our system we demonstrate compatibility with fast resonant scanning, the ability to acquire neuronal images, and the capability to image vasculature at deep locations (>1 mm) within the mouse cerebral cortex.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.448978