SHV-14, a novel β-lactamase variant in Klebsiella pneumoniae isolates from Nijmegen, The Netherlands

Four ceftazidime-resistant isolates of a Klebsiella pneumoniae strain were collected from intensive care unit patients in Nijmegen, The Netherlands. These isolates had TEM-29 and SHV-14 beta-lactamases. SHV-14 is a novel variant, with two substitutions compared with the sequence of SHV-1: Ile8Phe an...

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Veröffentlicht in:	Antimicrobial agents and chemotherapy 2001, Vol.45 (1), p.309-311
Hauptverfasser:	MEIFANG YUAN, HALL, Lucinda M. C, HOOGKAMP-KORSTANJE, Jaa, LIVERMORE, David M
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology beta-Lactamases - genetics beta-Lactamases - isolation & purification beta-Lactamases - metabolism Biological and medical sciences Fundamental and applied biological sciences. Psychology Kinetics Klebsiella Infections - microbiology Klebsiella pneumoniae Klebsiella pneumoniae - drug effects Klebsiella pneumoniae - enzymology Klebsiella pneumoniae - genetics Mechanisms of Resistance Metabolism. Enzymes Microbial Sensitivity Tests Microbiology Molecular Sequence Data Netherlands Netherlands, Nijmegen Plasmids - genetics SHV-14 protein
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Zusammenfassung:	Four ceftazidime-resistant isolates of a Klebsiella pneumoniae strain were collected from intensive care unit patients in Nijmegen, The Netherlands. These isolates had TEM-29 and SHV-14 beta-lactamases. SHV-14 is a novel variant, with two substitutions compared with the sequence of SHV-1: Ile8Phe and Arg43Ser. Its gene also had a silent C-->T mutation at nucleotide 481. The SHV-14 enzyme had slightly higher V(max) rates than SHV-1 for oxyimino-aminothiazolyl cephalosporins, but this activity was insufficient for the enzyme to count as an extended-spectrum beta-lactamase.
ISSN:	0066-4804 1098-6596
DOI:	10.1128/AAC.45.1.309-311.2001