Introduction of a second "Green Revolution" mutation into wheat via in planta CRISPR/Cas9 delivery

Introduction of a second "Green Revolution" mutation into wheat via in planta CRISPR/Cas9 delivery

Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction of a semidwarf1-orthologous mutation into an elite wheat variety.

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Bibliographische Detailangaben
Veröffentlicht in:Plant physiology (Bethesda) 2022-03, Vol.188 (4), p.1838-1842
Hauptverfasser: Kumagai, Yuya, Liu, Yuelin, Hamada, Haruyasu, Luo, Weifeng, Zhu, Jianghui, Kuroki, Misa, Nagira, Yozo, Taoka, Naoaki, Katoh, Etsuko, Imai, Ryozo
Format: Artikel
Sprache:eng
Schlagworte:
CRISPR-Cas Systems - genetics
Focus Issue on Gene Editing and its Applications
Gene Editing
Mutation - genetics
RNA, Guide, CRISPR-Cas Systems - genetics
Triticum - genetics
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Beschreibung
Zusammenfassung:Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction of a semidwarf1-orthologous mutation into an elite wheat variety.
ISSN:0032-0889
1532-2548
DOI:10.1093/plphys/kiab570