Developing and validating a modified enzyme linked immunosorbent assay method for detecting HEV IgG antibody from dried blood spot (DBS) samples in endemic settings

Developing and validating a modified enzyme linked immunosorbent assay method for detecting HEV IgG antibody from dried blood spot (DBS) samples in endemic settings

Serological analysis is an integral part of laboratory practice nowadays. The present study was aimed to develop and validate a modified Enzyme linked Immunosorbent Assay (ELISA) for determination of IgG antibody against Hepatitis E Virus (HEV) using dried blood spots (DBS) and corresponding plasma...

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Veröffentlicht in:Microbes and infection 2022-03, Vol.24 (2), p.104890-104890, Article 104890
Hauptverfasser: Sultana, Rosy, Bhuiyan, Taufiqur Rahman, Sathi, Afsana Shirin, Sharmin, Salma, Yeasmin, Sharmina, Uddin, Muhammad Ikhtear, Bhuiyan, Md Saruar, Mannoor, Kaiissar, Karim, Muhammad Manjurul, Zaman, K., Qadri, Firdausi
Format: Artikel
Sprache:eng
Schlagworte:
Dried Blood Spot Testing - methods
Enzyme-Linked Immunosorbent Assay - methods
Hepatitis E virus
Humans
Immunoglobulin G
Plasma
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Zusammenfassung:Serological analysis is an integral part of laboratory practice nowadays. The present study was aimed to develop and validate a modified Enzyme linked Immunosorbent Assay (ELISA) for determination of IgG antibody against Hepatitis E Virus (HEV) using dried blood spots (DBS) and corresponding plasma samples. A total of 65 samples (45 HEV patients, 20 healthy controls) were analyzed. DBS and plasma samples demonstrated equivalent optical densities for detecting anti-HEV IgG. A highly significant correlation was observed between plasma and DBS sample absorbances (R2 = 0.98; p 
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2021.104890