Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?
Experimental studies suggest that vitamin D receptor signaling may benefit the gut microbiome. In humans, whether vitamin D supplementation directly alters the gut microbiome is not well studied. To determine whether correcting vitamin D deficiency with cholecalciferol (vitamin D3, D3) or calcifedio...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2021-12, Vol.106 (12), p.3464-3476 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Shieh, Albert Lee, S Melanie Lagishetty, Venu Gottleib, Carter Jacobs, Jonathan P Adams, John S |
| description | Experimental studies suggest that vitamin D receptor signaling may benefit the gut microbiome. In humans, whether vitamin D supplementation directly alters the gut microbiome is not well studied.
To determine whether correcting vitamin D deficiency with cholecalciferol (vitamin D3, D3) or calcifediol (25-hydroxyvitamin D3, 25(OH)D3) changes gut microbiome composition.
18 adults with vitamin D deficiency (25-hydroxyvitamin D [25(OH)D] |
| doi_str_mv | 10.1210/clinem/dgab573 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8864755</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A702281941</galeid><sourcerecordid>A702281941</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-fa5b474c547cb435ac0ec61e1db7f9fb2699ecd4e442568d937499a294e628a3</originalsourceid><addsrcrecordid>eNptktFrFDEQxoMo9nr66qMEfPFl2ySbbDY-KMddawsVfTjEt5BNJneR7Kbd7Ar97025syqUeQhMfvMx880g9IaSM8ooObcxDNCfu53phKyfoQVVXFSSKvkcLQhhtFKS_ThBpzn_JIRyLuqX6KTmJZhiCzR_CzFNeDsGE3Hy-HuYTB8GvKmxGRxem2iDBxdSxCV7BSZO-_u_FN6ADzbAMOGVm-OUP-BNgoyvJ7zem2EHeNoDvgRb1L8EO6YupB4-vUIvvIkZXh_fJdpeXmzXV9XN18_X69VNZbmQU-WN6LjkVnBpO14LYwnYhgJ1nfTKd6xRCqzjwDkTTetULblShikODWtNvUQfD7K3c9eDs6XN0UR9O4bejPc6maD__xnCXu_SL922DZdCFIH3R4Ex3c2QJ92HbCFGM0Cas2ZCtEQRVqxfoncHdGci6DD4VBTtA65XkjDWlsXQQp09QZVw0AebhuJmyT9VULzLeQT_2D0l-uEC9OEC9PECSsHbf2d-xP-svP4Nf0qtyQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2558090257</pqid></control><display><type>article</type><title>Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Shieh, Albert ; Lee, S Melanie ; Lagishetty, Venu ; Gottleib, Carter ; Jacobs, Jonathan P ; Adams, John S</creator><creatorcontrib>Shieh, Albert ; Lee, S Melanie ; Lagishetty, Venu ; Gottleib, Carter ; Jacobs, Jonathan P ; Adams, John S</creatorcontrib><description>Experimental studies suggest that vitamin D receptor signaling may benefit the gut microbiome. In humans, whether vitamin D supplementation directly alters the gut microbiome is not well studied.
To determine whether correcting vitamin D deficiency with cholecalciferol (vitamin D3, D3) or calcifediol (25-hydroxyvitamin D3, 25(OH)D3) changes gut microbiome composition.
18 adults with vitamin D deficiency (25-hydroxyvitamin D [25(OH)D] <20 ng/mL) received 60 µg/day of D3 or 20 µg/day of 25(OH)D3 for 8 weeks. Changes in serum 25(OH)D, 1,25-diydroxyvitamin D (1,25(OH)2D), and 24,25-dihydroxyvitamin D (24,25(OH)2D) were assessed. We characterized composition of the fecal microbiota using 16S rRNA gene sequencing, and examined changes in α-diversity (Chao 1, Faith's Phylogenetic Diversity, Shannon Index), β-diversity (DEICODE), and genus-level abundances (DESeq2).
Vitamin D3 and 25(OH)D3 groups were similar. After 8 weeks of vitamin D3, mean 25(OH)D and 24,25(OH)2D increased significantly, but 1,25(OH)2D did not (25(OH)D: 17.8-30.1 ng/mL, P = .002; 24,25(OH)2D: 1.1 to 2.7 ng/mL, P =0.003; 1,25(OH)2D: 49.5-53.0 pg/mL, P = .9). After 8 weeks of 25(OH)D3, mean 25(OH)D, 24,25(OH)2D, and 1,25(OH)2D increased significantly (25(OH)D: 16.7-50.6 ng/mL, P < .0001; 24,25(OH)2D: 1.3-6.2 ng/mL, P = .0001; 1,25(OH)2D: 56.5-74.2 pg/mL, P = .05). Fecal microbial α-diversity and β-diversity did not change with D3 or 25D3 supplementation. Mean relative abundance of Firmicutes increased and mean relative abundance of Bacterioidetes decreased from baseline to 4 weeks, but returned to baseline by study completion. DESeq2 analysis did not confirm any statistically significant taxonomic changes.
In a small sample of healthy adults with vitamin D deficiency, restoration of vitamin D sufficiency with vitamin D3 or 25(OH)D3 did not lead to lasting changes in the fecal microbiota.</description><identifier>ISSN: 0021-972X</identifier><identifier>ISSN: 1945-7197</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/clinem/dgab573</identifier><identifier>PMID: 34343292</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Adolescent ; Adult ; Adults ; Alfacalcidol ; Analysis ; Biological diversity ; Biomarkers - blood ; Calcifediol ; Calcifediol - administration & dosage ; Cholecalciferol - administration & dosage ; Clinical ; Dietary Supplements ; Feces - microbiology ; Female ; Follow-Up Studies ; Gastrointestinal Microbiome ; Humans ; Male ; Microbiota (Symbiotic organisms) ; Phylogeny ; Pilot Projects ; Prognosis ; Vitamin D ; Vitamin D Deficiency - blood ; Vitamin D Deficiency - drug therapy ; Vitamin D Deficiency - microbiology ; Vitamin D Deficiency - pathology ; Vitamins - administration & dosage ; Young Adult</subject><ispartof>The journal of clinical endocrinology and metabolism, 2021-12, Vol.106 (12), p.3464-3476</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>COPYRIGHT 2021 Oxford University Press</rights><rights>The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-fa5b474c547cb435ac0ec61e1db7f9fb2699ecd4e442568d937499a294e628a3</citedby><cites>FETCH-LOGICAL-c457t-fa5b474c547cb435ac0ec61e1db7f9fb2699ecd4e442568d937499a294e628a3</cites><orcidid>0000-0002-0695-7976</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34343292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shieh, Albert</creatorcontrib><creatorcontrib>Lee, S Melanie</creatorcontrib><creatorcontrib>Lagishetty, Venu</creatorcontrib><creatorcontrib>Gottleib, Carter</creatorcontrib><creatorcontrib>Jacobs, Jonathan P</creatorcontrib><creatorcontrib>Adams, John S</creatorcontrib><title>Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Experimental studies suggest that vitamin D receptor signaling may benefit the gut microbiome. In humans, whether vitamin D supplementation directly alters the gut microbiome is not well studied.
To determine whether correcting vitamin D deficiency with cholecalciferol (vitamin D3, D3) or calcifediol (25-hydroxyvitamin D3, 25(OH)D3) changes gut microbiome composition.
18 adults with vitamin D deficiency (25-hydroxyvitamin D [25(OH)D] <20 ng/mL) received 60 µg/day of D3 or 20 µg/day of 25(OH)D3 for 8 weeks. Changes in serum 25(OH)D, 1,25-diydroxyvitamin D (1,25(OH)2D), and 24,25-dihydroxyvitamin D (24,25(OH)2D) were assessed. We characterized composition of the fecal microbiota using 16S rRNA gene sequencing, and examined changes in α-diversity (Chao 1, Faith's Phylogenetic Diversity, Shannon Index), β-diversity (DEICODE), and genus-level abundances (DESeq2).
Vitamin D3 and 25(OH)D3 groups were similar. After 8 weeks of vitamin D3, mean 25(OH)D and 24,25(OH)2D increased significantly, but 1,25(OH)2D did not (25(OH)D: 17.8-30.1 ng/mL, P = .002; 24,25(OH)2D: 1.1 to 2.7 ng/mL, P =0.003; 1,25(OH)2D: 49.5-53.0 pg/mL, P = .9). After 8 weeks of 25(OH)D3, mean 25(OH)D, 24,25(OH)2D, and 1,25(OH)2D increased significantly (25(OH)D: 16.7-50.6 ng/mL, P < .0001; 24,25(OH)2D: 1.3-6.2 ng/mL, P = .0001; 1,25(OH)2D: 56.5-74.2 pg/mL, P = .05). Fecal microbial α-diversity and β-diversity did not change with D3 or 25D3 supplementation. Mean relative abundance of Firmicutes increased and mean relative abundance of Bacterioidetes decreased from baseline to 4 weeks, but returned to baseline by study completion. DESeq2 analysis did not confirm any statistically significant taxonomic changes.
In a small sample of healthy adults with vitamin D deficiency, restoration of vitamin D sufficiency with vitamin D3 or 25(OH)D3 did not lead to lasting changes in the fecal microbiota.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Adults</subject><subject>Alfacalcidol</subject><subject>Analysis</subject><subject>Biological diversity</subject><subject>Biomarkers - blood</subject><subject>Calcifediol</subject><subject>Calcifediol - administration & dosage</subject><subject>Cholecalciferol - administration & dosage</subject><subject>Clinical</subject><subject>Dietary Supplements</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gastrointestinal Microbiome</subject><subject>Humans</subject><subject>Male</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Phylogeny</subject><subject>Pilot Projects</subject><subject>Prognosis</subject><subject>Vitamin D</subject><subject>Vitamin D Deficiency - blood</subject><subject>Vitamin D Deficiency - drug therapy</subject><subject>Vitamin D Deficiency - microbiology</subject><subject>Vitamin D Deficiency - pathology</subject><subject>Vitamins - administration & dosage</subject><subject>Young Adult</subject><issn>0021-972X</issn><issn>1945-7197</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptktFrFDEQxoMo9nr66qMEfPFl2ySbbDY-KMddawsVfTjEt5BNJneR7Kbd7Ar97025syqUeQhMfvMx880g9IaSM8ooObcxDNCfu53phKyfoQVVXFSSKvkcLQhhtFKS_ThBpzn_JIRyLuqX6KTmJZhiCzR_CzFNeDsGE3Hy-HuYTB8GvKmxGRxem2iDBxdSxCV7BSZO-_u_FN6ADzbAMOGVm-OUP-BNgoyvJ7zem2EHeNoDvgRb1L8EO6YupB4-vUIvvIkZXh_fJdpeXmzXV9XN18_X69VNZbmQU-WN6LjkVnBpO14LYwnYhgJ1nfTKd6xRCqzjwDkTTetULblShikODWtNvUQfD7K3c9eDs6XN0UR9O4bejPc6maD__xnCXu_SL922DZdCFIH3R4Ex3c2QJ92HbCFGM0Cas2ZCtEQRVqxfoncHdGci6DD4VBTtA65XkjDWlsXQQp09QZVw0AebhuJmyT9VULzLeQT_2D0l-uEC9OEC9PECSsHbf2d-xP-svP4Nf0qtyQ</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Shieh, Albert</creator><creator>Lee, S Melanie</creator><creator>Lagishetty, Venu</creator><creator>Gottleib, Carter</creator><creator>Jacobs, Jonathan P</creator><creator>Adams, John S</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0695-7976</orcidid></search><sort><creationdate>20211201</creationdate><title>Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?</title><author>Shieh, Albert ; Lee, S Melanie ; Lagishetty, Venu ; Gottleib, Carter ; Jacobs, Jonathan P ; Adams, John S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-fa5b474c547cb435ac0ec61e1db7f9fb2699ecd4e442568d937499a294e628a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Adults</topic><topic>Alfacalcidol</topic><topic>Analysis</topic><topic>Biological diversity</topic><topic>Biomarkers - blood</topic><topic>Calcifediol</topic><topic>Calcifediol - administration & dosage</topic><topic>Cholecalciferol - administration & dosage</topic><topic>Clinical</topic><topic>Dietary Supplements</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Gastrointestinal Microbiome</topic><topic>Humans</topic><topic>Male</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Phylogeny</topic><topic>Pilot Projects</topic><topic>Prognosis</topic><topic>Vitamin D</topic><topic>Vitamin D Deficiency - blood</topic><topic>Vitamin D Deficiency - drug therapy</topic><topic>Vitamin D Deficiency - microbiology</topic><topic>Vitamin D Deficiency - pathology</topic><topic>Vitamins - administration & dosage</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shieh, Albert</creatorcontrib><creatorcontrib>Lee, S Melanie</creatorcontrib><creatorcontrib>Lagishetty, Venu</creatorcontrib><creatorcontrib>Gottleib, Carter</creatorcontrib><creatorcontrib>Jacobs, Jonathan P</creatorcontrib><creatorcontrib>Adams, John S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shieh, Albert</au><au>Lee, S Melanie</au><au>Lagishetty, Venu</au><au>Gottleib, Carter</au><au>Jacobs, Jonathan P</au><au>Adams, John S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>106</volume><issue>12</issue><spage>3464</spage><epage>3476</epage><pages>3464-3476</pages><issn>0021-972X</issn><issn>1945-7197</issn><eissn>1945-7197</eissn><abstract>Experimental studies suggest that vitamin D receptor signaling may benefit the gut microbiome. In humans, whether vitamin D supplementation directly alters the gut microbiome is not well studied.
To determine whether correcting vitamin D deficiency with cholecalciferol (vitamin D3, D3) or calcifediol (25-hydroxyvitamin D3, 25(OH)D3) changes gut microbiome composition.
18 adults with vitamin D deficiency (25-hydroxyvitamin D [25(OH)D] <20 ng/mL) received 60 µg/day of D3 or 20 µg/day of 25(OH)D3 for 8 weeks. Changes in serum 25(OH)D, 1,25-diydroxyvitamin D (1,25(OH)2D), and 24,25-dihydroxyvitamin D (24,25(OH)2D) were assessed. We characterized composition of the fecal microbiota using 16S rRNA gene sequencing, and examined changes in α-diversity (Chao 1, Faith's Phylogenetic Diversity, Shannon Index), β-diversity (DEICODE), and genus-level abundances (DESeq2).
Vitamin D3 and 25(OH)D3 groups were similar. After 8 weeks of vitamin D3, mean 25(OH)D and 24,25(OH)2D increased significantly, but 1,25(OH)2D did not (25(OH)D: 17.8-30.1 ng/mL, P = .002; 24,25(OH)2D: 1.1 to 2.7 ng/mL, P =0.003; 1,25(OH)2D: 49.5-53.0 pg/mL, P = .9). After 8 weeks of 25(OH)D3, mean 25(OH)D, 24,25(OH)2D, and 1,25(OH)2D increased significantly (25(OH)D: 16.7-50.6 ng/mL, P < .0001; 24,25(OH)2D: 1.3-6.2 ng/mL, P = .0001; 1,25(OH)2D: 56.5-74.2 pg/mL, P = .05). Fecal microbial α-diversity and β-diversity did not change with D3 or 25D3 supplementation. Mean relative abundance of Firmicutes increased and mean relative abundance of Bacterioidetes decreased from baseline to 4 weeks, but returned to baseline by study completion. DESeq2 analysis did not confirm any statistically significant taxonomic changes.
In a small sample of healthy adults with vitamin D deficiency, restoration of vitamin D sufficiency with vitamin D3 or 25(OH)D3 did not lead to lasting changes in the fecal microbiota.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>34343292</pmid><doi>10.1210/clinem/dgab573</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-0695-7976</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adolescent Adult Adults Alfacalcidol Analysis Biological diversity Biomarkers - blood Calcifediol Calcifediol - administration & dosage Cholecalciferol - administration & dosage Clinical Dietary Supplements Feces - microbiology Female Follow-Up Studies Gastrointestinal Microbiome Humans Male Microbiota (Symbiotic organisms) Phylogeny Pilot Projects Prognosis Vitamin D Vitamin D Deficiency - blood Vitamin D Deficiency - drug therapy Vitamin D Deficiency - microbiology Vitamin D Deficiency - pathology Vitamins - administration & dosage Young Adult |
| title | Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome? |
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