Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Johns Hopkins All Children’s PREDICT Birth Cohort Study

Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Johns Hopkins All Children’s PREDICT Birth Cohort Study

Newborn screening using Dried Plasma Spots (DPS) offers pre-analytical advantages over conventional cards for plasma-associated targets of interest. Herein we present DPS-based methods for measuring metabolites using a 400+ compound LC-MS/MS library. Quality assurance reduced this library to 134, an...

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Veröffentlicht in:The Journal of pediatrics 2021-10, Vol.241, p.251-256.e4
Hauptverfasser: Schleif, William S., Harlan, Robert S., Hamblin, Frances, Amankwah, Ernest K., Goldenberg, Neil A., Hernandez, Raquel G., Johnson, Sara B., Reed, Shannon, Graham, David R.
Format: Artikel
Sprache:eng
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Zusammenfassung:Newborn screening using Dried Plasma Spots (DPS) offers pre-analytical advantages over conventional cards for plasma-associated targets of interest. Herein we present DPS-based methods for measuring metabolites using a 400+ compound LC-MS/MS library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.
ISSN:0022-3476
1097-6833
DOI:10.1016/j.jpeds.2021.09.061