Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates

Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates

Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast t...

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Veröffentlicht in:Journal of clinical microbiology 2001-07, Vol.39 (7), p.2683-2686
Hauptverfasser: BART-DELABESSE, Emmanuelle, SARFATI, Jacqueline, DEBEAUPUIS, Jean-Paul, VAN LEEUWEN, Willem, VAN BELKUM, Alex, BRETAGNE, Stephane, LATGE, Jean-Paul
Format: Artikel
Sprache:eng
Schlagworte:
Aspergillosis - microbiology
Aspergillus fumigatus
Aspergillus fumigatus - classification
Aspergillus fumigatus - genetics
Bacteriology
Biological and medical sciences
DNA Fingerprinting - methods
DNA, Fungal - analysis
DNA, Fungal - genetics
Environmental Microbiology
Epidemiology
Fundamental and applied biological sciences. Psychology
Human mycoses
Humans
Infectious diseases
Medical sciences
Microbiology
Microsatellite Repeats - genetics
Mycological Typing Techniques
Mycology
Mycoses
Mycoses of the respiratory system
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
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Zusammenfassung:Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.39.7.2683-2686.2001